Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection

文献信息

发布日期 2003-07-14

DOI 10.1039/B303949H

影响因子 4.616

作者

Qing Deng, Lisa M. Kauri, Wei-Jun Qian, Gabriella M. Dahlgren, Robert T. Kennedy

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摘要

A microscale method for purines involved in intracellular signaling and energy metabolism, including ADP, ATP, cyclic-AMP, NADH and GTP, was developed. The analytes were separated on a fused-silica capillary liquid chromatography column (50 µm inner diameter by 25 cm long) packed with 7 µm reversed-phase particles and detected with a carbon fiber cylinder microelectrode at +1.50 V versus Ag/AgCl reference electrode. With an acetonitrile gradient, the separation was carried out within 15 min. With a 100 nl injection volume, the detection limits varied from 0.9 to 8 fmol depending upon the analyte. The low detection limits make the method suitable for analysis of small tissue samples. As a demonstration of the method, islets of Langerhans were analyzed for their adenosine-related messenger content.

期刊推荐

Chinese Journal of Chemistry

Critical Reviews in Solid State and Materials Sciences

Main Group Chemistry

Medicinal Chemistry Research

Bioorganic & Medicinal Chemistry

Herald of the Russian Academy of Sciences

Topics in Catalysis

Journal of the Indian Institute of Science

NDT & E International

Journal of Chemical Sciences

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection

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