Hidden variety of biotin–streptavidin/avidin local interactions revealed by site-selective dynamic force spectroscopy
文献信息
Atsushi Taninaka, Osamu Takeuchi, Hidemi Shigekawa
By site-selective dynamic force spectroscopy realized with the combination of cross-linkers and anatomic force microscope with a force feedback system, we have revealed, for the first time, that the slight difference between the local structures of amino acid residues at the middle sites, SER45 and THR35 for streptavidin and avidin, respectively, strongly affects the microscopic reaction processes, i.e., the variation governs the type of bond as well as the fine structure of the potential landscape. For streptavidin, a bridged or direct hydrogen bond is induced depending on the molecular structure in the buffer solution. For avidin, in contrast, only a direct hydrogen bond is observed for all the buffer solutions used in the experiment. Since final functions in a system are realized through the assembly of local effects, the obtained results indicate the importance of analyzing the reaction processes with respect to the local structures of molecules, for further development of nanoscale functional devices.
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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.














