Functionalized nanoscale through microscale polypeptide stabilized emulsions for display of biomolecules
文献信息
发布日期
2011-03-15
DOI
10.1039/C1PY00045D
影响因子
5.582
作者
Jarrod A. Hanson
查看原文
摘要
We have prepared stable nano- and microscale emulsion droplets using block copolypeptide surfactants that have been functionalized with biotin. We have varied the biotin density on the droplet surfaces by mixing biotinylated with non-biotinylated surfactants, and used these to optimize the level of biotinylation for binding of NeutrAvidin. Using fluorescence microscopy and dynamic light scattering we have verified specific binding of NeutrAvidin to the biotinylated droplets and developed conditions for specific binding of biotinylated ligands to the NeutrAvidin coated droplets. These experiments outline a process for preparation of a soft, nano- through microscale sized droplet platform capable of specifically conjugating and displaying biotinylated molecules.
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来源期刊
Polymer Chemistry
CiteScore:
8.6
自引率:
7.3%
年发文量:
457
Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.
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