![methyl 6-amino-1H-pyrrolo[2,3-b]pyridine-4-carboxylate structure](https://cnstatic.chemtradehub.com/structs/119/1190315-60-6-9d9a.webp "methyl 6-amino-1H-pyrrolo[2,3-b]pyridine-4-carboxylate structure")
Covalently incorporated protein–nanogels using AGET ATRP in an inverse miniemulsion
文献信息
发布日期
2011-03-24
DOI
10.1039/C1PY00050K
影响因子
5.582
作者
Saadyah E. Averick, Andrew J. D. Magenau, Antonina Simakova, Bradley F. Woodman, Andrew Seong, Ryan A. Mehl, Krzysztof Matyjaszewski
查看原文
摘要
Using a genetically engineered protein, containing a non-natural amino acid bearing an atom transfer radical polymerization (ATRP) initiator, protein–nanogel hybrids (PNHs) were synthesized by activator generated by electron transfer (AGET) ATRP in an inverse miniemulsion. The route presented is an appropriate synthetic strategy to covalently and site specifically incorporate green fluorescent protein (GFP) into well-defined nanogels. These PNHs were analyzed using dynamic light scattering (DLS), UV-visible fluorescence spectroscopy and confocal microscopy to confirm the successful integration of GFP proteins into each nanogel (NG), while preserving its native tertiary structure.
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来源期刊
Polymer Chemistry
CiteScore:
8.6
自引率:
7.3%
年发文量:
457
Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.
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