On-line visualization of multicolor chemical images with stimulated Raman scattering spectral microscopy
文献信息
发布日期
2015-03-30
DOI
10.1039/C5AN00335K
影响因子
4.616
作者
Yoichi Otsuka, Koji Makara, Shuya Satoh, Hiroyuki Hashimoto, Yasuyuki Ozeki
查看原文
摘要
We demonstrate multicolor, on-line visualization in label-free biomedical microscopy based on stimulated Raman scattering (SRS). Fast data acquisition of SRS spectral images and subsequent image generation are achieved. The loading vectors for the blind separation of chemical components are predetermined by multivariate analysis at a certain field of view (FOV) and are applied to execute on-line visualization of chemical images at other FOVs. We also show that the response time can be shortened by reducing the number of spectral data points. This on-line visualization system is expected to increase the usability of the Raman imaging system and the analytical throughput for screening.
期刊推荐
Current Opinion in Colloid & Interface Science
Russian Journal of General Chemistry
Organic Process Research & Development
Russian Journal of Bioorganic Chemistry
Russian Journal of Applied Chemistry
Journal of Natural Medicines
New Journal of Chemistry
Current Opinion in Solid State & Materials Science
Russian Journal of Organic Chemistry
Acta Materialia
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来源期刊
Analyst
CiteScore:
7.8
自引率:
5.6%
年发文量:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
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