![Imidazo[1,2-c]pyrimidine structure](https://cnstatic.chemtradehub.com/structs/274/274-78-2-8b4c.webp "Imidazo[1,2-c]pyrimidine structure")
PCR free multiple ligase reactions and probe cleavages for the SNP detection of KRAS mutation with attomole sensitivity
文献信息
发布日期
2016-10-19
DOI
10.1039/C6AN00909C
影响因子
4.616
作者
查看原文
摘要
A method to produce multiple ligated primers without PCR for a target DNA containing a single point mutation is presented. A strand displacing hairpin was introduced into the reaction, enabling separation of the ligated primer target DNA duplex without any thermal denaturing process. The ligated product was cycled to allow cleavage of fluorescence labeled substrates for RNase H on gold nanoparticles, leading to target specific fluorescence amplification. As a result, 10 attomoles of target DNA containing a point mutation in the KRAS gene were detected.
期刊推荐
Advanced Engineering Materials
Molecules
Foundations of Chemistry
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Angewandte Chemie International Edition
Molecular Diversity
Environmental Toxicology and Pharmacology
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Journal of Enzyme inhibition and Medicinal Chemistry
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来源期刊
Analyst
CiteScore:
7.8
自引率:
5.6%
年发文量:
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Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
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