![4-[(1-Methyl-1H-pyrrol-2-yl)methylene]-1,3(2H,4H)-isoquinolinedione structure](https://cnstatic.chemtradehub.com/structs/110/1104546-89-5-a600.webp "4-[(1-Methyl-1H-pyrrol-2-yl)methylene]-1,3(2H,4H)-isoquinolinedione structure")
4-[(1-Methyl-1H-pyrrol-2-yl)methylene]-1,3(2H,4H)-isoquinolinedione
CAS号:
1104546-89-5
分子式:
C15H12N2O2
Simple and rapid detection of bacteria using a nuclease-responsive DNA probe
文献信息
发布日期
2017-11-24
DOI
10.1039/C7AN01384A
影响因子
4.616
作者
Jeong Moon, Kyoungsook Park
查看原文
摘要
We demonstrate simple and rapid bacterial detection using a nuclease-responsive DNA probe. The probe consisting of a fluorescent dye and a quencher at the 5′ and 3′ termini, respectively, was designed to be cleaved by nucleases such as endonucleases, exonucleases, and DNases, which are released from bacteria using an optimized lysis buffer. The fluorescence signal of the cleaved DNA probe correlates with the number of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, and the detection limit was 103 CFU for E. coli and 104 CFU for S. aureus. Moreover, this method is specific for live bacteria and takes just one minute to get the signal including sample collection. These features make the present bacterial detection method a powerful on-site bacterial contamination assay which is simple, rapid, and quantitative.
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来源期刊
Analyst
CiteScore:
7.8
自引率:
5.6%
年发文量:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
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