![2-{3-[4-(3-Chlorophenyl)-1-piperazinyl]propyl}[1,2,4]triazolo[4,3-a]pyridin-3(2H)-one hydrochloride (1:1) structure](https://cnstatic.chemtradehub.com/structs/253/25332-39-2-496e.webp "2-{3-[4-(3-Chlorophenyl)-1-piperazinyl]propyl}[1,2,4]triazolo[4,3-a]pyridin-3(2H)-one hydrochloride (1:1) structure")
Efficient synthesis and replication of diverse sequence libraries composed of biostable nucleic acid analogues
文献信息
发布日期
2022-08-30
DOI
10.1039/D2CB00035K
影响因子
0
作者
John R. D. Hervey, Niklas Freund, Gillian Houlihan, Gurpreet Dhaliwal, Philipp Holliger, Alexander I. Taylor
查看原文
摘要
Functional nucleic acids can be evolved in vitro using cycles of selection and amplification, starting from diverse-sequence libraries, which are typically restricted to natural or partially-modified polymer chemistries. Here, we describe the efficient DNA-templated synthesis and reverse transcription of libraries entirely composed of serum nuclease resistant alternative nucleic acid chemistries validated in nucleic acid therapeutics; locked nucleic acid (LNA), 2′-O-methyl-RNA (2′OMe-RNA), or mixtures of the two. We evaluate yield and diversity of synthesised libraries and measure the aggregate error rate of a selection cycle. We find that in addition to pure 2′-O-methyl-RNA and LNA, several 2′OMe-RNA/LNA blends seem suitable and promising for discovery of biostable functional nucleic acids for biomedical applications.
期刊推荐
相关文献
Kinetic evaluation of chitosan-derived catalysts for the aldol reaction in water
Anton De Vylder, Jeroen Lauwaert, Jeriffa De Clercq, Pascal Van Der Voort, Christian V. Stevens, Joris W. Thybaut
2019-08-29
Paper
DOI: 10.1039/C9RE00245F
Electrochemical production of syngas from CO2 at pressures up to 30 bar in electrolytes containing ionic liquid
Sofia Messias, Miguel M. Sousa, Manuel Nunes da Ponte, Carmen M. Rangel, Tiago Pardal, Ana S. Reis Machado
2019-07-31
Paper
DOI: 10.1039/C9RE00271E
Kinetic study of esterification over structured ZSM-5-coated catalysts based on fluid flow situations in macrocellular foam materials
Xin Gao, Qiuyan Ding, Yan Wu, Yilai Jiao, Jinsong Zhang, Xingang Li, Hong Li
2020-01-20
Paper
DOI: 10.1039/C9RE00445A
Microfluidic surface-enhanced infrared spectroscopy with semiconductor plasmonics for the fingerprint region
Mario Bomers, Benoît Charlot, Franziska Barho, Antoine Chanuel, Aude Mezy, Laurent Cerutti, Fernando Gonzalez-Posada, Thierry Taliercio
2019-11-15
Paper
DOI: 10.1039/C9RE00350A
In situ synthesis of gold nanoparticles in polymer films under concentrated sunlight: control of nanoparticle size and shape with solar flux
L. Peres, V. Goetz, M. Respaud, K. Soulantica
2019-12-17
Paper
DOI: 10.1039/C9RE00439D
Elementary reaction pathway study and a deduced macrokinetic model for the unified understanding of Ni-catalyzed steam methane reforming
Changming Ke, Zijing Lin
2020-02-26
Paper
DOI: 10.1039/C9RE00460B
Jet-mixing reactor for the production of monodisperse silver nanoparticles using a reduced amount of capping agent
Pinaki Ranadive, Aamena Parulkar, Nicholas A. Brunelli
2019-05-14
Paper
DOI: 10.1039/C9RE00152B
Living lamellar crystal initiating polymerization and brittleness mechanism investigations based on crystallization during the ring-opening of cyclic butylene terephthalate oligomers
Zongbao Wang, Bingjie Wang, Quting Gou, Junwu Zhang, Jian Zhou, Peng Chen, Qun Gu
2012-12-03
Paper
DOI: 10.1039/C2PY20847D
Highly efficient asymmetric reduction of ketopantolactone to d-(−)-pantolactone by Escherichia coli cells expressing recombinant conjugated polyketone reductase and glucose dehydrogenase in a fed-batch biphasic reaction system
Jiapao Wang, Haoteng Zheng, Pengfei Cheng, Yifeng Wu, Anming Wang, Weike Su
2020-01-08
Paper
DOI: 10.1039/C9RE00385A
您可能还喜欢
化合物问答
4-[4-三氟甲基苯基]恶唑(CAS号：1126636-40-5)通常如何合成？
4-[4-三氟甲基苯基]恶唑通常通过将4-三氟甲基苯酚与异硫氰酸苯酯在有机溶剂中进行酯化反应合成。该反应可在无水条件下，使用适当的催化剂，如四丁基氢氧化铵，以提...
1126636-40-54-(4-(Trifluoromethy...
59729-32-71-[3-(Dimethylamino)...
化合物问答
RockPhos Pd G3(CAS号：2009020-38-4)通常如何合成？
RockPhos Pd G3 通常通过钯催化偶联反应合成，使用配体 (2'-Amino-2-biphenylyl)(methanesulfonato-kappa...
2009020-38-4(2'-Amino-2-biphenyl...
化合物问答
1-哌啶甲酰胺(CAS号：2158-03-4)的市场或研究趋势如何？
1-哌啶甲酰胺作为有机合成中的重要中间体，其市场需求主要受医药、农药、染料等行业推动。近年来，随着新药开发和绿色化学的发展，该化合物的研究趋势集中在开发更高效、...
2158-03-41-Piperidinecarboxam...
化合物问答
2-(二苯基膦基)乙胺(CAS号：4848-43-5)适用哪些法规指南？
2-(二苯基膦基)乙胺适用于多种法规指南，包括但不限于《全球化学品统一分类和标签制度》（GHS），欧盟《化学品注册、评估、授权和限制》法规（REACH），以及美...
4848-43-52-(Diphenylphosphino...
化合物问答
如何储存间苯二甲酸二烯丙酯(CAS号：1087-21-4)？
间苯二甲酸二烯丙酯应储存在阴凉、干燥、通风良好的地方，远离火源和热源。储存容器应密封，避免光照和高温。储存温度应控制在25℃以下，相对湿度应低于80%。避免与其...
1087-21-4Diallyl isophthalate
化合物问答
什么是间甲苯异硫代异氰酸酯(CAS号：621-30-7)？
间甲苯异硫代异氰酸酯是一种有机化合物，分子式为C7H7NO2S，具有刺激性气味。它是一种重要的有机合成中间体，在合成其他化合物时广泛应用。
621-30-71-Isothiocyanato-3-m...
化合物问答
在合成中是否有N-Boc-D-苯丙氨醇(CAS号：106454-69-7)的替代品？
在合成中，可以考虑使用N-Cbz-D-苯丙氨醇或N-Fmoc-D-苯丙氨醇作为替代品。这些化合物同样具有保护氨基的功能，且在合成过程中表现出良好的反应性能。
106454-69-72-Methyl-2-propanyl ...
化合物问答
3-羟甲基-2-氧异丙基吡啶(CAS号：954240-50-7)的主要用途是什么？
3-羟甲基-2-氧异丙基吡啶主要用于有机合成领域，可以作为合成其他药物、农药或精细化学品的中间体。此外，它还可能在实验室研究中作为特定反应的前体或溶剂。
954240-50-7(2-Isopropoxy-3-pyri...
化合物问答
6-氨基-9-甲基嘌呤(CAS号：700-00-5)应用于哪些行业？
6-氨基-9-甲基嘌呤目前主要应用于医药行业，作为某些药物的中间体。此外，它还可能用于聚合物、传感器和半导体的某些领域，作为功能性单体或掺杂剂。
700-00-59-Methyl-9H-purin-6-...
来源期刊
推荐化合物
![[2',6'-bis(propan-2-yloxy)-[1,1'-biphenyl]-3-yl]dicyclohexylphosphane structure](https://cnstatic.chemtradehub.com/structs/787/787618-22-8-dda2.webp "[2',6'-bis(propan-2-yloxy)-[1,1'-biphenyl]-3-yl]dicyclohexylphosphane structure")
[2',6'-bis(propan-2-yloxy)-[1,1'-biphenyl]-3-yl]dicyclohexylphosphane
CAS号:
787618-22-8
分子式:
C30H43O2P
推荐供应商
免责声明
本页面提供的学术期刊信息仅供参考和研究使用。我们与任何期刊出版商均无关联，也不处理投稿事宜。如有投稿相关咨询，请直接联系相关期刊出版商。
如发现页面信息有误，请发送邮件至 support@chemtradehub.com 联系我们。我们将及时核实并处理您的问题。