Development of a modularized aptamer targeting the nuclear T-cell suppressor PAC1

文献信息

发布日期 2023-05-16

DOI 10.1039/D3AN00011G

影响因子 4.616

作者

Zixi Hu, Zhongyu Jiang, Zeliang Yang, Liang Liu, Zhenyu Zhu, Yan Jin, Yuxin Yin

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摘要

Aptamers associated with cancer targeting therapy are commonly focused on cell membrane proteins; however, the study of intracellular, particularly, nuclear proteins is limited. The nuclear phosphatase PAC1 has been reported to be a potential T cell-related immunotherapeutic target. Here, we identified an aptamer, designated as PA5, with high affinity and specificity for PAC1 through the systematic evolution of ligands by exponential enrichment (SELEX) procedure. We then developed a dual-module aptamer PAC1-AS consisting of a cell-internalizing module and a targeting module, which can recognize PAC1 in the nucleus under physiological conditions. This modularized aptamer raises the possibility of manipulating endosomes and provides insights into the exploration and development of an efficient cancer immunotherapy approach.

期刊推荐

Australian Journal of Chemistry

Journal of the Chinese Chemical Society

Accounts of Chemical Research

Analyst

Chemistry of Natural Compounds

Corrosion Science

Anti-Corrosion Methods and Materials

Bulletin of the Chemical Society of Japan

Ferroelectrics

Canadian Metallurgical Quarterly

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Development of a modularized aptamer targeting the nuclear T-cell suppressor PAC1

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期刊推荐

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