Excited-state dynamics and fluorescence lifetime of cryogenically cooled green fluorescent protein chromophore anions
文献信息
Anne P. Rasmusssen, Henrik B. Pedersen, Lars H. Andersen
Time-resolved action spectroscopy together with a fs-pump probe scheme is used in an electrostatic ion-storage ring to address lifetimes of specific vibrational levels in electronically excited states. Here we specifically consider the excited-state lifetime of cryogenically cooled green fluorescent protein (GFP) chromophore anions which is systematically measured across the S0–S1 spectral region (450–482 nm). A long lifetime of 5.2 ± 0.3 ns is measured at the S0–S1 band origin. When exciting higher vibrational levels in S1, the lifetime changes dramatically. It decreases by more than two orders of magnitude in a narrow energy region ∼250 cm−1 (31 meV) above the 0–0 transition. This is attributed to the opening of internal conversion over an excited-state energy barrier. The applied experimental technique provides a new way to uncover even small energy barriers, which are crucial for excited-state dynamics.
相关文献
Molecular dynamics simulation for insight into microscopic mechanism of polymer reinforcement
Sizhu Wu, Liqun Zhang, Wenchuan Wang
DOI: 10.1039/C0CP00297F
Molecular dynamics simulation of surface segregation in a (110) B2-NiAl thin film
Elena V. Levchenko, Alexander V. Evteev, Rafal Kozubski, Irina V. Belova, Graeme E. Murch
DOI: 10.1039/C0CP00376J
Chiral polymerization: symmetry breaking and entropy production in closed systems
Celia Blanco, David Hochberg
DOI: 10.1039/C0CP00992J
State-to-state inelastic scattering of Stark-decelerated OH radicals with Ar atoms
Ludwig Scharfenberg, Jacek Kłos, Paul J. Dagdigian, Millard H. Alexander, Gerard Meijer, Sebastiaan Y. T. van de Meerakker
DOI: 10.1039/C004422A
Switching of functionalized azobenzene suspended between gold tips by mechanochemical, photochemical, and opto-mechanical means
Robert Turanský, Martin Konôpka, Nikos L. Doltsinis, Ivan Štich, Dominik Marx
DOI: 10.1039/C0CP00588F
Micellar transitions in the aqueous solutions of a surfactant-like ionic liquid: 1-butyl-3-methylimidazolium octylsulfate
Tejwant Singh, Markus Drechsler, Axel H. E. Müeller, I. Mukhopadhyay, Arvind Kumar
DOI: 10.1039/C003855P
Dissociative recombination of the acetaldehyde cation, CH3CHO+
Mathias Hamberg, Vitali Zhaunerchyk, Magdalena Kaminska, Richard D. Thomas, Sebastian Trippel, Iryna Kashperka, Magnus af Ugglas, Catherine Walsh, Roland Wester, Jacek Semaniak, Mats Larsson, Wolf D. Geppert
DOI: 10.1039/C003857A
Synthesis and characterization of hybrid organic/inorganic nanotubes of the imogolite type and their behaviour towards methane adsorption
Barbara Bonelli, Sharon E. Ashbrook, Paul A. Wright, Wuzong Zhou, Marco Tagliabue, Marco Armandi, Edoardo Garrone
DOI: 10.1039/C0CP00438C
119Sn MAS NMR and first-principles calculations for the investigation of disorder in stannate pyrochlores
Martin R. Mitchell, Simon W. Reader, Karen E. Johnston, Chris J. Pickard, Karl R. Whittle, Sharon E. Ashbrook
DOI: 10.1039/C0CP01274B
Functionalization vs. fragmentation: n-aldehydeoxidation mechanisms and secondary organic aerosol formation
Heber J. Chacon-Madrid, Albert A. Presto, Neil M. Donahue
DOI: 10.1039/C0CP00200C
您可能还喜欢
N-2,2-丙烯基-2-丙烯酰胺(CAS号:2555-13-7)通常如何合成?
N-2,2-丙烯基-2-丙烯酰胺通常通过丙烯酰胺与丙烯基卤化物的缩合反应合成。该反应通常在温和的条件下进行,使用适量的碱如吡啶作为催化剂。反应的选择性良好,产率...
什么是1,2-二碘四氟代乙烷(CAS号:354-65-4)?
1,2-二碘四氟代乙烷是一种有机化合物,化学式为C2F4I2,CAS号为354-65-4。它是一种无色透明液体,具有特殊的化学性质和物理性质,包括高沸点、低挥发...
3-溴-1H-吡咯[3,2-c]吡啶-4-碳腈(CAS号:1000341-71-8)适用哪些法规指南?
根据GHS(全球化学品统一分类和标签制度),3-溴-1H-吡咯[3,2-c]吡啶-4-碳腈被归类为第2类易燃液体。在欧盟,该化合物需要符合REACH法规的要求,...
1-氯甲基萘磺酸(CAS号:87491-79-0)安全吗?
1-氯甲基萘磺酸在使用时需要谨慎,因为它具有一定的刺激性和腐蚀性。操作时应佩戴适当的防护装备,如防化服、手套、护目镜等,避免直接接触皮肤和吸入其蒸汽。
二氯(二环戊二烯)铂(CAS号:12083-92-0)的主要用途是什么?
该化合物主要用于催化剂领域,特别是在有机合成中的催化氧化反应以及作为某些药物合成的中间体。此外,它还被研究用于纳米材料的制备。
3-溴-7-氯噻吩并[3,2-b]吡啶-6-甲腈(CAS号:798574-82-0)安全吗?
3-溴-7-氯噻吩并[3,2-b]吡啶-6-甲腈在处理时需要谨慎,因其含有溴和氯等强卤素,可能具有一定的刺激性和腐蚀性。使用时应佩戴适当的个人防护装备,避免皮肤...
(R)-1-((R)-2-(2’-二环己基膦苯基)三戊铁基]乙基(双-3,5-三氟甲基苯基)膦(CAS号:494227-32-6)的主要用途是什么?
该化合物主要用于有机合成领域,特别是作为催化剂或配体,在有机合成反应中发挥重要作用。此外,它还可能应用于催化加氢反应、偶联反应等。
3-[6-(Diphenylphosphoryl)-2-naphthyl]-1,10-phenanthroline(CAS号:1480371-38-7)安全吗?
3-[6-(Diphenylphosphoryl)-2-naphthyl]-1,10-phenanthroline在正常使用条件下相对安全,但在操作时应穿戴适当...
在合成中是否有ETHYL 2-(4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)CYCLOHEX-3-ENYL)ACETATE(CAS号:1166829-70-4)的替代品?
可以考虑使用类似结构的化合物作为替代品,如2-(4-环戊基环己烯基)乙酸酯,这种化合物在结构上相似,可能在某些合成路径中作为替代品。
如何处理含有3-(3-氨基丙基)丙酮缩甘油(CAS号:131606-42-3)的废料?
处理含有3-(3-氨基丙基)丙酮缩甘油的废料时,首先应确保遵守当地的环保法规。对于危险废物,应进行分类收集,然后送至专业的废物处理设施进行焚烧或安全填埋。在处理...
来源期刊
Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.










![Methyl 2-[5-(3-Phenoxyphenyl)-2H-tetrazol-2-yl]acetate structure Methyl 2-[5-(3-Phenoxyphenyl)-2H-tetrazol-2-yl]acetate structure](https://cnstatic.chemtradehub.com/structs/130/1305320-60-8-84b4.webp)
![5-Bromo-3-isopropyl-1H-pyrrolo[2,3-b]pyridine structure 5-Bromo-3-isopropyl-1H-pyrrolo[2,3-b]pyridine structure](https://cnstatic.chemtradehub.com/structs/125/1256819-54-1-8620.webp)


