May GSH and l-His contribute to intracellular binding of zinc? Thermodynamic and solution structural study of a ternary complex

文献信息

发布日期 2003-02-21
DOI 10.1039/B300632H
影响因子 6.222
作者

Artur Krężel, Jacek Wójcik, Maciej Maciejczyk, Wojciech Bal


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摘要

GSH and L-His are abundant biomolecules and likely biological ligands for Zn(II) under certain conditions. Potentiometric titrations provide evidence of formation of ternary Zn(II) complexes with GSH and L-His or D-His with slight stereoselectivity in favour of L-His (ca. 1 log unit of stability constant). The solution structure of the ZnH(GSH)(L-His)(H2O) complex at pH 6.8, determined by NMR, includes tridentate L-His, monodentate (sulfur) GSH, and weak interligand interactions. Calculations of competitivity of this complex for Zn(II) binding at pH 7.4 indicate that it is likely to be formed in vivo under conditions of GSH depletion. Otherwise, GSH alone emerges as a likely Zn(II) carrier.

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Chemical Communications

Chemical Communications
CiteScore: 8.6
自引率: 4.7%
年发文量: 2458

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