Gene sensors based on peptide nucleic acid (PNA) probes: Relationship between sensor sensitivity and probe/target duplex stability

文献信息

发布日期 2005-08-02

DOI 10.1039/B507121F

影响因子 4.616

作者

Hiroshi Aoki, Hiroaki Tao

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摘要

Gene sensors based on peptide nucleic acid (PNA) probes were prepared and the relationship between sensor sensitivity and the duplex stability of the probe PNAs and target complementary DNAs was studied using five synthesized PNAs (10-, 15-, 17-, 20-, and 22-mers). It was found that the association constants for the probe PNA/target DNA pairs depend not only on the length but also on the base pair sequence, and that the trend in the sensor responses was the same as that in the association constants for the corresponding pairs. In addition, by using two kinds of probe PNAs with different lengths, it was demonstrated that fabrication of sensors based on probe PNAs with comparable association constants yielded similar response curves and sensor sensitivities.

期刊推荐

Journal of Chemical Sciences

Bioorganic & Medicinal Chemistry

Acta Metallurgica Sinica-English Letters

Journal of Asian Natural Products Research

Atomization and Sprays

Medicinal Chemistry Research

Chinese Journal of Chemistry

Herald of the Russian Academy of Sciences

Electroanalysis

Cellulose

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Gene sensors based on peptide nucleic acid (PNA) probes: Relationship between sensor sensitivity and probe/target duplex stability

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