Antibody–gold quantum dot–PAMAM dendrimer complex as an immunoglobulin immunoassay
文献信息
Robert C. Triulzi, Miodrag Micic, Jhony Orbulescu, Silvia Giordani, Bill Mueller, Roger M. Leblanc
Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.
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