Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiOx interfaces

文献信息

发布日期 2008-07-01

DOI 10.1039/B804825H

影响因子 4.616

作者

Maël Manesse, Valerie Stambouli, Rabah Boukherroub

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摘要

The use of Au/SiOx interfaces for the investigation of DNA hybridization using electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) simultaneously is demonstrated. Standard glass chemistry was used to link single-stranded DNA (ss-DNA) on aldehyde-terminated Au/SiOx interfaces. The layer thickness and amount of grafted oligonucleotides (ODNs) were calculated from SPR on the basis of a multilayer system of glass/Ti/Au/SiOx/grafted molecule. Capacitance and resistance values of the modified interface before and after hybridization were calculated from EIS data using an equivalent circuit and allowed the affinity rate constant, KA = 4.07 × 105 M−1, to be determined. The EIS results were comparable to those obtained by SPR hybridization kinetics recorded in parallel.

期刊推荐

Critical Reviews in Solid State and Materials Sciences

Colloid Journal

NDT & E International

Bioorganic & Medicinal Chemistry

Cellulose

Journal of Asian Natural Products Research

Medicinal Chemistry Research

Topics in Catalysis

Journal of the Indian Institute of Science

Electroanalysis

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiOx interfaces

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