Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements

文献信息

发布日期 2009-04-27

DOI 10.1039/B822070K

影响因子 4.616

作者

Ilkka Lähdesmäki, Petr Chocholouš, Andrea D. Carroll, Judy Anderson, Peter S. Rabinovitch, Jaromir Ruzicka

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摘要

This work introduces, for the first time, simultaneous monitoring of fluorescence and absorbance using Bead Injection in a Lab-on-valve format. The aim of the paper is to show that when the target species, cells immobilized on a stationary phase, are exposed to reagents under well-controlled reaction conditions, dual monitoring yields valuable information. The applicability of this technique is demonstrated by the development of a Bead Injection method for automated measurement of cell density and intracellular hydrogen peroxide.

期刊推荐

Critical Reviews in Solid State and Materials Sciences

Biocatalysis and Biotransformation

Bioorganic & Medicinal Chemistry

Acta Metallurgica Sinica-English Letters

Medicinal Chemistry Research

Atomization and Sprays

Journal of the Indian Institute of Science

Chinese Journal of Chemistry

Herald of the Russian Academy of Sciences

Heteroatom Chemistry

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements

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