Engineering poly(lactic-co-glycolic acid)/calcium carbonate microspheres with controllable topography and their cell response

文献信息

发布日期 2013-05-30
DOI 10.1039/C3TB20284D
影响因子 6.331
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摘要

Polymeric porous microspheres can be used as functional vehicles in drug delivery and cell culture. However, the conventional porous microspheres are somewhat complicated with respect to preparation, and limited in composition and controllability. Here we report the preparation of poly(lactic-co-glycolic acid)/calcium carbonate (PLGA/CC) composite microspheres with uniform superficial macropores through a facile single-step method, where CC acts simultaneously as in situ pore-forming agent and reinforcing phase. The SEM images and quantified results from mercury intrusion porosimetry indicated that the size and density of the superficial macropores were highly controllable via changing the starting parameters such as size and content of CC particles and concentration of PLGA. Mouse bone mesenchymal stem cells were cultured on microspheres of different topographies to investigate the cell–substrate interaction. The results showed that cells adhered and grew well on all microspheres, while the topography with smaller and more discrete macropores exhibited the highest proliferation, indicating that cells responded to the topography of the microsphere. This work provides a novel approach to obtain diverse porous composite microspheres designed for tissue repair and study of cell–substrate interaction.

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来源期刊

Journal of Materials Chemistry B

Journal of Materials Chemistry B
CiteScore: 12
自引率: 4.9%
年发文量: 831

Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. The journals have a strong history of publishing quality reports of interest to interdisciplinary communities and providing an efficient and rigorous service through peer review and publication. The journals are led by an international team of Editors-in-Chief and Associate Editors who are all active researchers in their fields. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C. More than one Journal of Materials Chemistry journal may be suitable for certain fields and researchers are encouraged to submit their paper to the journal that they feel best fits for their particular article. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive. Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices image block All articles published in Journal of Materials Chemistry B from 2019 onwards will be indexed in MEDLINE®. Articles that primarily focus on providing insight into the underlying science and performance of biomaterials within a biological environment are more suited to our companion journal, Biomaterials Science.

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