Direct immobilization of glucose oxidase in magnetic mesoporous bioactive glasses

文献信息

发布日期 2013-05-13
DOI 10.1039/C3TB20480D
影响因子 6.331
作者

Dandan Min, Xudong Zhang, Wen He, Yang Zhang, Piwu Li, Meimei Zhang, Jianan Liu, Shujiang Liu, Fengxiu Xu, Yi Du, Zhilong Zhang


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摘要

Direct immobilization of enzymes on the bioactive glasses is conceptually a completely new strategy. We find that the Fe2O3–CaO–SiO2–P2O5 magnetic mesoporous bioactive glass (MMBG) is an ideal immobilization matrix for glucose oxidase (GOD). Its unique chemical surface properties and open mesopores enhance the catalytic activity of directly immobilized GOD. In this paper, MMBG was synthesized using the sol–gel approach and polyethylene glycol (PEG) as template at 700 °C. GOD molecules were spontaneously entrapped inside the open mesoporous structure and onto the surface of MMBG via iron ion binding, their activity was not impaired. The substrates and products can access and diffuse freely through the open mesoporous structure in MMBG. This study is focused on understanding the formation mechanism of MMBG, the immobilized mechanism of GOD and the magnetic separation mechanism of MMBG from the reaction medium. The MMBG can be utilized in the design of a solid support for any enzyme for bioconversion, bioremediation, and biosensors.

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来源期刊

Journal of Materials Chemistry B

Journal of Materials Chemistry B
CiteScore: 12
自引率: 4.9%
年发文量: 831

Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. The journals have a strong history of publishing quality reports of interest to interdisciplinary communities and providing an efficient and rigorous service through peer review and publication. The journals are led by an international team of Editors-in-Chief and Associate Editors who are all active researchers in their fields. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C. More than one Journal of Materials Chemistry journal may be suitable for certain fields and researchers are encouraged to submit their paper to the journal that they feel best fits for their particular article. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive. Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices image block All articles published in Journal of Materials Chemistry B from 2019 onwards will be indexed in MEDLINE®. Articles that primarily focus on providing insight into the underlying science and performance of biomaterials within a biological environment are more suited to our companion journal, Biomaterials Science.

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