Predicting efficacies of anticancer drugs using single cell HaloChip assay

文献信息

发布日期 2016-02-29

DOI 10.1039/C5AN02564H

影响因子 4.616

作者

Yong Qiao, Jincui An

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摘要

Single cell halo assay (HaloChip) is used to quantify DNA repair ability and predict the efficacy of anticancer drugs. After exposure to drugs, cells are patterned onto a substrate to form an ordered single cell array, then embedded inside an agarose gel, and fluorescently stained to generate a characteristic halo surrounding each cell. The extent of DNA repair is quantified by using a relative nuclear diffusion factor (rNDF) derived from the surface areas of nuclei and halos. Several repair-competent and repair-deficient cell lines have been used to validate this method. Drug-inhibitor combinations are also tested in the context of synthetic lethality of chemotherapy, where the use of a repair inhibitor potentiates the effects of DNA damaging agents. This paper highlights the important role of HaloChip in quantifying DNA repair ability, which provides the diagnostic utility to enhance the efficacies of anticancer drugs.

期刊推荐

Lab on a Chip

Photochemical & Photobiological Sciences

CrystEngComm

Molecular Diversity

Physical Chemistry Chemical Physics

Current Pharmaceutical Biotechnology

Advanced Engineering Materials

Angewandte Chemie International Edition

Foundations of Chemistry

Molecules

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Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Predicting efficacies of anticancer drugs using single cell HaloChip assay

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