Access to aliphatic protons as reporters in non-deuterated proteins by solid-state NMR
文献信息
Suresh Kumar Vasa, Petra Rovó, Karin Giller, Stefan Becker, Rasmus Linser
Interactions within proteins, with their surrounding, and with other molecules are mediated mostly by hydrogen atoms. In fully protonated, inhomogeneous, or larger proteins, however, aliphatic proton shifts tend to show little dispersion despite fast Magic-Angle Spinning. 3D correlations dispersing aliphatic proton shifts by their better resolved amide N/H shifts can alleviate this problem. Using inverse second-order cross-polarization (iSOCP), we here introduce dedicated and improved means to sensitively link site-specific chemical shift information from aliphatic protons with a backbone amide resolution. Thus, even in cases where protein deuteration is impossible, this approach may enable access to various aspects of protein functions that are reported on by protons.
相关文献
Aerobic conversion of benzylic sp3 C–H in diphenylmethanes and benzyl ethers to CO bonds under catalyst-, additive- and light-free conditions
Xinqiang Cheng, Xinzheng Yang, Yun-Lai Ren, Kaisheng Yao, Huiyong Wang, Jianji Wang
DOI: 10.1039/C9QO00004F
Transition-metal-free oxidative reaction of hydrazines and potassium metabisulfite for preparation of sulfonohydrazides
Yang Wang, Bingnan Du, Wanxing Sha, Haibo Mei, Jianlin Han, Yi Pan
DOI: 10.1039/C7QO00168A
I2-Catalyzed sulfenylation of indoles and pyrroles using triethylammonium thiolates as sulfenylating agents
Wei Fan, Zhen Yang
DOI: 10.1039/C6QO00851H
The solvent effect on the excited-state intramolecular proton transfer of cyanine derivative molecules
Wei Shi, Yunfan Yang, Yu Zhao, Yongqing Li
DOI: 10.1039/C9QO00230H
Endo/exo binding of alkyl and aryl diammonium ions by cyclopentanocucurbit[6]uril
Yun-Xia Qu, Rui-Lian Lin, Yun-Qian Zhang, Kai-Zhi Zhou, Qing-Di Zhou, Qian-Jiang Zhu, Zhu Tao, Pei-Hua Ma, Jing-Xin Liu, Gang Wei
DOI: 10.1039/C7QO00376E
(E)-Selective Friedel–Crafts acylation of alkynes to β-chlorovinyl ketones: defying isomerizations in batch reactions by flow chemistry approaches
Hyungmo Koo, Hun Young Kim, Kyungsoo Oh
DOI: 10.1039/C9QO00217K
[Ag(bpy)(PPhtBu2)(OCF3)]: a stable nucleophilic reagent for chemoselective and stereospecific trifluoromethoxylation of secondary alkyl nosylates
Daoqian Chen, Long Lu, Qilong Shen
DOI: 10.1039/C9QO00278B
Direct synthesis of hydrazones by visible light mediated aerobic oxidative cleavage of the CC bond
Ya Ding, Hao Li, Yunge Meng, Te Zhang, Jiawen Li, Qiu-Yun Chen
DOI: 10.1039/C7QO00276A
Visible light driven, nickel-catalyzed aryl esterification using a triplet photosensitiser thioxanthen-9-one
Da-Liang Zhu, Hong-Xi Li, Ze-Ming Xu, Hai-Yan Li, David J. Young, Jian-Ping Lang
DOI: 10.1039/C9QO00536F
您可能还喜欢
4,5-二甲基-2-硝基苯甲酸(CAS号:4315-14-4)的市场或研究趋势如何?
4,5-二甲基-2-硝基苯甲酸主要应用于制药、染料和农药等行业。由于其潜在的毒性,其市场趋势可能受到法规限制和环保考量的影响,推动了替代产品的研发。在研究领域,...
处理直接黑22(CAS号:6473-13-8)时应注意哪些实验室安全事项?
处理直接黑22时应穿戴适当的个人防护装备(PPE),包括实验服、手套、护目镜和口罩。操作应在通风橱内进行,以避免吸入有害气体。如果发生泄漏,应立即清理,并使用大...
处理2,1,3-苯并噻二唑-4-基异氰酸酯(CAS号:342411-14-7)时应注意哪些实验室安全事项?
处理2,1,3-苯并噻二唑-4-基异氰酸酯时应注意以下安全事项:穿戴个人防护装备,如实验室外套、防护眼镜和手套;在通风橱中操作,确保良好的通风;保持实验室环境干...
如何处理含有Δ-8,9-脱氢雌酮(CAS号:204077-66-7)的废料?
含有Δ-8,9-脱氢雌酮的废料需要进行适当的处理以确保环境和人体安全。首先,收集废液并存放于密封容器中,避免泄漏。其次,可以考虑将其转化为无害物质或通过专业处理...
如何储存5-溴戊酸(CAS号:2067-33-6)?
5-溴戊酸应储存在阴凉、干燥、通风良好的环境中,避免阳光直射。建议在室温(约15-25°C)下保存,保持相对湿度低于60%。应使用密封的玻璃或塑料容器,并远离热...
4-(甲基亚磺酰基)苯胺(CAS号:22865-62-9)应用于哪些行业?
4-(甲基亚磺酰基)苯胺在医药、聚合物和传感器等领域有一定的应用。在医药方面,它可以用作合成药物的中间体;在聚合物领域,可以作为合成特殊性能高分子材料的单体;在...
什么是1-(2-FLUOROPHENYL)-5-METHYL-1H-PYRAZOLE-4-CARBOHYDRAZIDE(CAS号:618092-58-3)?
1-(2-氟苯基)-5-甲基-1H-吡唑-4-亚甲基肼是一种有机化合物,其分子式为C9H9FN3O。该化合物具有特定的物理化学性质,如熔点、沸点等,但具体值需查...
Dauricumine(CAS号:345641-00-1)通常如何合成?
Dauricumine通常通过复杂的合成路线制备,涉及多个步骤,包括环化、氧化、卤化等反应。合成过程中使用了多种催化剂和试剂,例如金属催化剂、氧化剂等。产率通常...
5-氰基苯酞(CAS号:82104-74-3)安全吗?
5-氰基苯酞在正常使用条件下相对安全,但其具有一定的毒性,需谨慎操作。在实验或工业应用中,应采取适当的防护措施,如佩戴防护手套、护目镜和实验服,确保通风良好。误...
2-Methyl-1-(phenylsulfonyl)-1H-pyrrolo[2,3-b]pyridin-5-amine(CAS号:1186502-59-9)安全吗?
该化合物在使用时需要谨慎操作。虽然其毒性和健康风险尚未完全明确,但建议在通风良好的环境中操作,并穿戴适当的个人防护装备,如手套和防护眼镜。
来源期刊
Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.














