A fully battery-powered inexpensive spectrophotometric system for high-sensitivity point-of-care analysis on a microfluidic chip
文献信息
发布日期
2016-04-20
DOI
10.1039/C6AN00370B
影响因子
4.616
作者
Maowei Dou, Juan Lopez, Misael Rios, Oscar Garcia, Chuan Xiao, Michael Eastman, XiuJun Li
查看原文
摘要
A cost-effective ttery-powered pectrophotometric ystem (BASS) was developed for quantitative point-of-care (POC) analysis on a microfluidic chip. By using methylene blue as a model analyte, we first compared the performance of the BASS with a commercial spectrophotometric system, and further applied the BASS for loop-mediated isothermal amplification (LAMP) detection and subsequent quantitative nucleic acid analysis which exhibited a comparable limit of detection to that of Nanodrop. Compared to the commercial spectrophotometric system, our spectrophotometric system is lower-cost, consumes less reagents, and has higher detection sensitivity. Most importantly, it does not rely on external power supplies. All these features make our spectrophotometric system highly suitable for a variety of POC analyses, such as field detection.
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Russian Journal of Organic Chemistry
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