Electrochemical detection of protein glycosylation using lectin and protein–gold affinity interactions

文献信息

发布日期 2016-03-11

DOI 10.1039/C6AN00528D

影响因子 4.616

作者

Laura G. Carrascosa, Abu A. I. Sina, Muhammad J. A. Shiddiky

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摘要

We report a new method for the electrochemical detection of glycosylation on proteins, which relies on lectin–protein interaction on a bare gold electrode. The target protein isolated by immunoaffinity is directly adsorbed onto a gold surface and its glycosylation status is retrieved by subsequent addition of specific lectins. The adsorption and subsequent recognition process is monitored electrochemically in the presence of [Fe(CN)6]3−/4− redox system. By decoupling target protein capture from glycosylation read-out steps, this approach circumvents unwanted antibody–lectin crosstalk while enabling specific glycosylation detection of a glycoprotein in serum-spiked samples in less than 1 h.

期刊推荐

European Journal of Organic Chemistry

Lab on a Chip

Environmental Toxicology and Pharmacology

Journal of Medical Biochemistry

Nature Reviews Drug Discovery

Current Pharmaceutical Biotechnology

Advanced Engineering Materials

Photochemical & Photobiological Sciences

CrystEngComm

Mini-Reviews in Medicinal Chemistry

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Electrochemical detection of protein glycosylation using lectin and protein–gold affinity interactions

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