Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification
文献信息
发布日期
2018-02-12
DOI
10.1039/C8CC00540K
影响因子
6.222
作者
Fei Ma, Wen-jing Liu, Li Liang, Bo Tang, Chun-Yang Zhang
查看原文
摘要
We develop a new fluorescence method for the sensitive detection of alkaline phosphatase (ALP) based on dephosphorylation-initiated transcription reaction-mediated dual signal amplification. This research demonstrates for the first time a significant improvement in ALP assay performance by using a nucleic acid amplification strategy, which enables sensitive detection of ALP activity in vitro and in cell extracts. Importantly, this method can be further applied for kinetic analysis and ALP inhibition assay.
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来源期刊
Chemical Communications
CiteScore:
8.6
自引率:
4.7%
年发文量:
2458
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