DNA-templated copper nanoclusters obtained via TdT isothermal nucleic acid amplification for mercury(ii) assay
文献信息
发布日期
2019-07-17
DOI
10.1039/C9AY01214A
影响因子
2.896
作者
Jing-Lin He, Xing-Xing Wang, Ting-Ting Mei, Ju-Lan Zeng, Jian-Hui Wang, Jianxiu Wang, Zhong Cao
查看原文
摘要
Herein, DNA-templated copper nanoclusters (CuNCs) synthesized by terminal deoxynucleotidyl transferase (TdT) isothermal nucleic acid amplification (INAA) were proposed for Hg2+ assay. TdT recognized the 3′-OH end of primers and subsequently prolonged the single strand poly-T at 3′-OH with the assistance of deoxythymidine triphosphate (dTTP). The generated products coordinated with the copper ions to form DNA-templated CuNCs, resulting in remarkable fluorescence. The introduction of Hg2+ made the primers form a T–Hg2+–T mismatch duplex, which prevented the capture of the 3′-OH end by TdT. The poly-T single strand prolongation reaction was suspended; this led to fluorescence quenching. Hg2+ could be detected at a concentration as low as 76 pM with a significant linear range from 0.2 to 500 nM. This method, performed in one tube in one step, does not involve washing and separation steps, which provides a novel insight into the ultra-sensitive detection of toxic analytes in water quality applications.
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来源期刊
Analytical Methods
CiteScore:
5.1
自引率:
3.7%
年发文量:
655
Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development
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