Viscosity sensitive endoplasmic reticulum fluorescent probes based on oxazolopyridinium

文献信息

发布日期 2021-06-09
DOI 10.1039/D1TB01106E
影响因子 6.331
作者

Ya-Nan Wang, Xiao-Qing Zhang, Ru Sun, Yu-Jie Xu, Jian-Feng Ge


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摘要

A series of viscosity sensitive fluorescent probes 1a–e were synthesized by linking coumarin and oxazolopyridinium via dimethylene in this paper. The viscosity test of probes 1a–e indicated that the fluorescence intensity of the probes enhanced significantly with the increase of viscosity of the system (0.89–865 cP), and exhibited a nearly OFF–ON response to viscosity at 648 nm, 650 nm and 650 nm, respectively. In addition, cells still had a high survival rate after co-culturing with probes 1a–e for 12 h (94–98%). Meanwhile, the laser confocal experiment showed that the variation of the carbon chain length in the oxazolopyridinium could affect the subcellular region of the localization of the probes in cells. When the length of the carbon chain in oxazolopyridinium was between n-C7H15 and n-C12H23, probes 1b–d had the ability to target the endoplasmic reticulum in the cells. Moreover, probes 1b–d showed no significant change in fluorescence intensity after 35 min of continuous laser confocal irradiation, indicating that they had excellent anti-photobleaching properties.

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来源期刊

Journal of Materials Chemistry B

Journal of Materials Chemistry B
CiteScore: 12
自引率: 4.9%
年发文量: 831

Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. The journals have a strong history of publishing quality reports of interest to interdisciplinary communities and providing an efficient and rigorous service through peer review and publication. The journals are led by an international team of Editors-in-Chief and Associate Editors who are all active researchers in their fields. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C. More than one Journal of Materials Chemistry journal may be suitable for certain fields and researchers are encouraged to submit their paper to the journal that they feel best fits for their particular article. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive. Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices image block All articles published in Journal of Materials Chemistry B from 2019 onwards will be indexed in MEDLINE®. Articles that primarily focus on providing insight into the underlying science and performance of biomaterials within a biological environment are more suited to our companion journal, Biomaterials Science.

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