In-source fragmentation of nucleosides in electrospray ionization towards more sensitive and accurate nucleoside analysis

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发布日期 2023-02-21

DOI 10.1039/D3AN00047H

影响因子 4.616

作者

Yu-Nan Chen, Xu-Yang Shen, Yue Yu, Chen-Yu Xue, Ying-Lin Zhou, Xin-Xiang Zhang

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摘要

Nucleosides have been found to suffer in-source fragmentation (ISF) in electrospray ionization mass spectrometry, which leads to reduced sensitivity and ambiguous identification. In this work, a combination of theoretical calculations and nuclear magnetic resonance analysis revealed the key role of protonation at N3 near the glycosidic bond during ISF. Therefore, an ultrasensitive liquid chromatography-tandem mass spectrometry system for 5-formylcytosine detection was developed with 300 fold signal enhancement. Also, we established a MS1-only platform for nucleoside profiling and successfully identified sixteen nucleosides in the total RNA of MCF-7 cells. Taking ISF into account, we can realize analysis with higher sensitivity and less ambiguity, not only for nucleosides, but for other molecules with similar protonation and fragmentation behaviors.

期刊推荐

Chemical & Pharmaceutical Bulletin

Cement and Concrete Research

Doklady Chemistry

Canadian Metallurgical Quarterly

Australian Journal of Chemistry

Biopolymers

Carbon

Analyst

AIAA Journal

Journal of the Chinese Chemical Society

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Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

In-source fragmentation of nucleosides in electrospray ionization towards more sensitive and accurate nucleoside analysis

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