A new type of reusable piezoimmunosensor fabricated by a recombinant IgG-binding protein

文献信息

发布日期 2000-02-24

DOI 10.1039/A909736H

影响因子 4.616

作者

Xuezhen Ren, Eiry Kobatake, Masuo Aizawa

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摘要

A reusable immunosensor was developed with the self-assembly of a recombinant antibody-binding protein on the gold electrode of a quartz piezoelectric crystal as the antibody receptor. The recombinant protein, B5C1, was genetically produced by introducing a cysteine residue at the C-terminus of 5 B-domains of protein A. Its self-assembling properties were studied using piezoelectric quartz crystals. The B5C1 assembled crystal showed higher antibody- binding activity and was more stable than that of the native protein A coated crystal. One B5C1 assembled crystal can be used repeatedly at least 30 times without detectable loss of antibody-binding activity.

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Journal of Asian Natural Products Research

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Herald of the Russian Academy of Sciences

Journal of the Indian Institute of Science

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Bioorganic & Medicinal Chemistry Letters

Bioorganic & Medicinal Chemistry

Chinese Journal of Chemistry

Heteroatom Chemistry

Polycyclic Aromatic Compounds

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

A new type of reusable piezoimmunosensor fabricated by a recombinant IgG-binding protein

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