UV-PEDD photometry dedicated for bioanalytical uses

文献信息

发布日期 2009-03-13
DOI 10.1039/B822025E
影响因子 4.616
作者

Łukasz Tymecki, Luiza Brodacka, Beata Rozum, Robert Koncki


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摘要

Detection of p-nitrophenol, a product of several enzymatic reactions, is useful and important in many fields of modern bioanalytics. Here, we have developed and applied a complete photometer dedicated for such measurements that is extremely economic and easily customized for specific experimental setups. The instrument consists only of paired emitter–detector diodes (PEDDs) coupled with an extremely simple and low-cost signal transduction system. The experimental data confirmed that this photometric system adapted for cuvette as well as flow measurements can be applied for fast and reproducible determination of alkaline phosphatase (ALP) activity.

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