Facile analysis of metabolites by capillary electrophoresis coupled to matrix-assisted laser desorption/ionization mass spectrometry using target plates with polysilazane nanocoating and grooves

文献信息

发布日期 2009-05-27

DOI 10.1039/B907039G

影响因子 4.616

作者

Andrea Amantonico, Pawel L. Urban, Renato Zenobi

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摘要

We present an inexpensive method and apparatus for the deposition of analytes separated by capillary electrophoresis (CE) onto a custom-designed matrix-assisted laser desorption/ionization (MALDI) target plate. This dedicated CE-MALDI plate is coated with an omniphobic polysilazane nanocoating and has an array of parallel grooves acting as recipients of the separation effluent. The 3-D pattern in the top layer of the coated plate greatly improves loading of the matrix solution prior to separation and facilitates deposition of the separated species. We demonstrate application of this straightforward protocol to the analysis of metabolites from the central metabolic pathway in a complex biological sample spiked with small molecule standards.

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Herald of the Russian Academy of Sciences

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Facile analysis of metabolites by capillary electrophoresis coupled to matrix-assisted laser desorption/ionization mass spectrometry using target plates with polysilazane nanocoating and grooves

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