Identification of a thermally activated process in the Cy3 photobleaching mechanism

文献信息

发布日期 2011-12-21
DOI 10.1039/C2CP22334A
影响因子 3.676
作者

A. L. Jouonang, P. Didier, Y. Mély


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摘要

Single molecule experiments were used to investigate the photobleaching mechanism of single Cy3 molecules encapsulated in large unilamellar vesicles. Analysis of thousands of individual fluorescence traces evidenced that the survival probability distribution is monoexponential with an average survival time depending on the laser intensity, the concentration of molecular oxygen, the temperature and the rigidity of the medium. Taken together, our data suggest that the photobleaching of Cy3 molecules is governed by two parallel pathways, namely photooxidation and a thermally activated structural rearrangement of the Cy3 molecules in their excited state.

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来源期刊

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
自引率: 10.3%
年发文量: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

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