Design and application of an open tubular capillary reactor for solid-phase permethylation of glycans in glycoprotein

文献信息

发布日期 2014-12-24

DOI 10.1039/C4AN01045K

影响因子 4.616

作者

Xiaodi Gao, Lingyi Zhang, Weibing Zhang, Liang Zhao

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摘要

The permethylation derivatization method for structural analysis of glycans is important for characterizing glycoproteins in the study of glycomics. An open tubular capillary reactor coated with NaOH was designed and constructed for solid-phase permethylation of glycans in glycoproteins. The flow rate, capillary length, and inner diameter of the reactor were optimized. The permethylation rate of the model sample β-cyclodextrin reached 81% with a flow rate of 1 μL min−1 in a 32 cm long capillary reactor (i.d. 500 μm). A trace amount of mucin O-glycans was permethylated by the open tubular reactor under low pressure without interference from the freezing of DMSO. Analysis indicates that using this open tubular reactor is a fast, convenient, and efficient method for the permethylation of protein O-glycan.

期刊推荐

Bulletin of the Chemical Society of Japan

Carbon

Anti-Corrosion Methods and Materials

Cement and Concrete Research

Journal of the Chinese Chemical Society

Advances in Colloid and Interface Science

Australian Journal of Chemistry

Accounts of Chemical Research

Doklady Chemistry

Biopolymers

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Design and application of an open tubular capillary reactor for solid-phase permethylation of glycans in glycoprotein

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