Design and application of an open tubular capillary reactor for solid-phase permethylation of glycans in glycoprotein

文献信息

发布日期 2014-12-24

DOI 10.1039/C4AN01045K

影响因子 4.616

作者

Xiaodi Gao, Lingyi Zhang, Weibing Zhang, Liang Zhao

查看原文

摘要

The permethylation derivatization method for structural analysis of glycans is important for characterizing glycoproteins in the study of glycomics. An open tubular capillary reactor coated with NaOH was designed and constructed for solid-phase permethylation of glycans in glycoproteins. The flow rate, capillary length, and inner diameter of the reactor were optimized. The permethylation rate of the model sample β-cyclodextrin reached 81% with a flow rate of 1 μL min−1 in a 32 cm long capillary reactor (i.d. 500 μm). A trace amount of mucin O-glycans was permethylated by the open tubular reactor under low pressure without interference from the freezing of DMSO. Analysis indicates that using this open tubular reactor is a fast, convenient, and efficient method for the permethylation of protein O-glycan.

期刊推荐

Angewandte Chemie International Edition

Environmental Toxicology and Pharmacology

Mini-Reviews in Medicinal Chemistry

Faraday Discussions

European Journal of Organic Chemistry

Advanced Engineering Materials

Foundations of Chemistry

Molecular Diversity

Nature Reviews Drug Discovery

Contact Lens & Anterior Eye

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Design and application of an open tubular capillary reactor for solid-phase permethylation of glycans in glycoprotein

文献信息

期刊推荐

相关文献

Natural products with 1,2-oxazine scaffold: occurrence, chemical diversity, bioactivity, synthesis, and biosynthesis

Contents list

Nanostructured metal oxide heterojunctions for chemiresistive gas sensors

Estimation and photocatalytic reduction of toxic chromium metal ions from environmental samples by zinc-based nanocomposite

Synthesis of three-component Cu2O/ZnO/Ag nanocrystal heterogeneous photocatalysts with high reactivity and stability for dye reduction

Electron transfer between neptunium and sodium chlorite in acidic chloride media

The preparation and performance evaluation of flame-retardant antistatic composites based on polyurethane

Mg-doped cathodic properties and solid-state ionic conduction in P2-type layered material for Na-ion batteries and supercapacitors

Construction of a metal–oxide interface through alloy nanoparticles to enhance CO2 electrolysis

A convenient synthetic approach to highly hindered 3,3′-bis(2,4,6-tri-tert-butylphenyl)-BINOL-derived phosphoric acids

您可能还喜欢

4-[[6-（3-苯基苯基）-7H-嘌呤-2-基]氨基]苯磺酰胺(CAS号：2079895-42-2)适用哪些法规指南？

反式-度骨化醇(CAS号：74007-20-8)的物理化学性质是什么？

莲花掌苷(CAS号：59282-56-3)的市场或研究趋势如何？

2-溴-6-(吡咯烷-1-基)吡啶-4-硼酸频那醇酯(CAS号：1150271-64-9)应用于哪些行业？

什么是methyl 2-(4-bromophenyl)-3-methylbutanoate(CAS号：1061284-70-5)？

CJC1-295(CAS号：863288-34-0)的物理化学性质是什么？

三正丁基锍碘(CAS号：18146-62-8)的市场或研究趋势如何？

雌二醇-[13C3]同位素内标(CAS号：1261254-48-1)通常如何合成？

N1-(2-吡啶甲基)-N2-(2-甲基-1-萘基)草酰胺(CAS号：2611225-93-3)的物理化学性质是什么？

如何处理含有十五碳烯酸甲酯(顺-10)(C15:1)标准品(CAS号：90176-52-6)的废料？

来源期刊

Analyst

推荐化合物

4-Chloro-2-{[(2-chlorophenoxy)acetyl]amino}benzoic acid

Cis-4-aminoadamantan-1-ol hydrochloride

tert-Butyl 6-chloro-4-oxospiro[chroman-2,4'-piperidine]-1'-carboxylate

(S)-(9H-Fluoren-9-yl)methyl (pyrrolidin-2-ylmethyl)carbamate hydrochloride

1-(Aminomethyl)cycloheptanol hydrochloride (1:1)

推荐供应商