Design of thiol–ene photoclick hydrogels using facile techniques for cell culture applications
文献信息
Lisa A. Sawicki
Thiol–ene ‘click’ chemistries have been widely used in biomaterials applications, including drug delivery, tissue engineering, and controlled cell culture, owing to their rapid, cytocompatible, and often orthogonal reactivity. In particular, hydrogel-based biomaterials formed by photoinitiated thiol–ene reactions afford spatiotemporal control over the biochemical and biomechanical properties of the network for creating synthetic materials that mimic the extracellular matrix or enable controlled drug release. However, the use of charged peptides functionalized with cysteines, which can form disulfides prior to reaction, and vinyl monomers that require multistep syntheses and contain ester bonds, may lead to undesired inhomogeneity or degradation under cell culture conditions. Here, we designed a thiol–ene hydrogel formed by the reaction of allyloxycarbonyl-functionalized peptides and thiol-functionalized poly(ethylene glycol). Hydrogels were polymerized by free radical initiation under cytocompatible doses of long wavelength ultraviolet light in the presence of water-soluble photoinitiators (lithium acylphosphinate, LAP, and 2-hydroxy-1-[4-(2-hydroxyethoxy)phenyl]-2-methyl-1-propanone, Irgacure 2959). Mechanical properties of these hydrogels were controlled by varying the monomer concentration to mimic a range of soft tissue environments, and hydrogel stability in cell culture medium was observed over weeks. Patterns of biochemical cues were created within the hydrogels post-formation and confirmed through the incorporation of fluorescently-labeled peptides and Ellman's assay to detect free thiols. Human mesenchymal stem cells remained viable after encapsulation and subsequent photopatterning, demonstrating the utility of the monomers and hydrogels for three-dimensional cell culture. This facile approach enables the formation and characterization of hydrogels with well-defined, spatially-specific properties and expands the suite of monomers available for three-dimensional cell culture and other biological applications.
期刊推荐
相关文献
Chemically-synthesised, atomically-precise gold clusters deposited and activated on titania
David P. Anderson, Jason F. Alvino, Alexander Gentleman, Hassan Al Qahtani, Lars Thomsen, Matthew I. J. Polson, Gregory F. Metha, Vladimir B. Golovko, Gunther G. Andersson
DOI: 10.1039/C3CP44005B
The importance of the TiO2/quantum dots interface in the recombination processes of quantum dot sensitized solar cells
Zion Tachan, Idan Hod, Menny Shalom, Larissa Grinis, Arie Zaban
DOI: 10.1039/C3CP44719G
Theoretical studies on the transport mechanism of 5-fluorouracil through cyclic peptide based nanotubes
Sofie Van Damme, Patrick Bultinck, Venkatesan Subramanian
DOI: 10.1039/C2CP42038D
Interactions in different domains of truxenone supramolecular assembly on Au(111)
Fengyun Chen, Zhenpeng Hu, Yongfei Ji, Aidi Zhao, Bing Wang, Jinlong Yang, J. G. Hou
DOI: 10.1039/C2CP23190E
Ultrafast dynamics in iron tetracarbonyl olefin complexes investigated with two-dimensional vibrational spectroscopy
Matthijs R. Panman, Arthur C. Newton, Jannie Vos, Bart van den Bosch, Vladica Bocokić, Joost N. H. Reek, Sander Woutersen
DOI: 10.1039/C2CP43565A
Understanding the different activities of highly promiscuous MbtI by computational methods
Silvia Ferrer, Sergio Martí, Vicent Moliner, Iñaki Tuñón, Juan Bertrán
DOI: 10.1039/C2CP23149B
Local thermal elevation probing of metal nanostructures during laser illumination utilizing surface-enhanced Raman scattering from a single-walled carbon nanotube
Shinji Hoshina, Masanobu Nara, Kei-ichiro Komeda, Ryukou Shito, Satoshi Yasuda, Kei Murakoshi
DOI: 10.1039/C3CP43728K
Photoinduced ultrafast dynamics of the triphenylamine-based organic sensitizer D35 on TiO2, ZrO2 and in acetonitrile
Kawon Oum, Peter W. Lohse, Johannes R. Klein, Oliver Flender, Mirko Scholz, Anders Hagfeldt, Gerrit Boschloo, Thomas Lenzer
DOI: 10.1039/C3CP44095H
Thermoelectric power factor optimization in PEDOT:PSS tellurium nanowire hybrid composites
Arun Majumdar, Jeffrey J. Urban
DOI: 10.1039/C3CP44558E
Room temperature ionic liquid as solvent for in situ Pd/H formation: hydrogenation of carbon–carbon double bonds
Benjamin C. M. Martindale, Dzianis Menshykau, Sven Ernst, Richard G. Compton
DOI: 10.1039/C2CP43444J
您可能还喜欢
4-((4-甲基哌嗪-1-基)甲基)苯硼酸(CAS号:763120-62-3)的市场或研究趋势如何?
随着有机硼化学的发展,该化合物在催化、药物合成、材料科学等领域展现出潜在的应用价值。近年来,其在药物前体合成中的应用越来越受到关注。市场趋势显示,随着科研投入的...
如何储存2,4,5-三甲基-1-硝基苯(CAS号:610-91-3)?
2,4,5-三甲基-1-硝基苯应储存在阴凉、干燥且通风良好的地方,避免阳光直射。储存在密封的金属容器中,远离火源和热源。储存温度应控制在25°C以下,湿度不宜过...
处理2,5-二碘噻吩(CAS号:625-88-7)时应注意哪些实验室安全事项?
在处理2,5-二碘噻吩时,应穿戴适当的个人防护装备(PPE),包括实验室外套、手套和防护眼镜。在通风橱中进行操作以避免吸入蒸气。如果发生泄漏,应立即疏散人员并使...
在合成中是否有6-bromo-3-chloro-1H-indole(CAS号:57916-08-2)的替代品?
在合成6-溴-3-氯-1H-吲哚(CAS号:57916-08-2)时,可以考虑使用一些类似的化合物作为替代品,如6-氯-3-氯-1H-吲哚或3-氯-1H-吲哚,...
在合成中是否有(R)-(-)-1-(1-萘基)乙基异氰酸酯(CAS号:42340-98-7)的替代品?
可以考虑使用类似结构的化合物,如1-[(1R)-1-(2-氨基乙基)萘-1-基]乙基异氰酸酯作为替代品。此外,还可以寻找其他类型的异氰酸酯衍生物,如苯基异氰酸酯...
3-氨基苯甲酰苯胺(CAS号:14315-16-3)适用哪些法规指南?
3-氨基苯甲酰苯胺适用于多项法规指南,包括但不限于GHS(全球化学品统一分类和标签制度)分类为皮肤腐蚀/刺激类别2,以及潜在的皮肤过敏性类别1。在欧盟地区,它受...
β-环柠檬醛-D5(CAS号:26309-95-5)通常如何合成?
β-环柠檬醛-D5可通过不对称合成方法获得。常见的合成路线包括以环己酮为原料,经过选择性氧化、还原、保护基引入等步骤,最终得到目标化合物。该合成过程中通常使用多...
如何处理含有BIO-1211(CAS号:187735-94-0)的废料?
对于含有BIO-1211(CAS号:187735-94-0)的废料,首先应进行分类收集,确保符合环保要求。然后,可以考虑通过焚烧或其他专业处理方法进行处置。在处...
如何处理含有4-氯-2-氟-3-甲基苯酚(CAS号:1351668-24-0)的废料?
含有该化合物的废液应收集至专用容器中,避免与其他化学品混合。可采用焚烧或送交专业废弃物处理公司处理。处理过程中需遵守当地环保法规,确保不产生二次污染。处理前应进...
来源期刊
Biomaterials Science

Biomaterials Science is an international high impact journal exploring the science of biomaterials and their translation towards clinical use. Its scope encompasses new concepts in biomaterials design, studies into the interaction of biomaterials with the body, and the use of materials to answer fundamental biological questions. Papers do not necessarily need to report a new biomaterial but should provide novel insight into the biological applications of the biomaterial. Articles that primarily focus on demonstrating novel materials chemistry and bring a molecular picture to bear on a given material’s suitability as a biomaterial are more suited to our companion journal, Journal of Materials Chemistry B. Biomaterials Science publishes primary research and review-type articles in the following areas: molecular design of biomaterials, including translation of emerging chemistries to biomaterials science of cells and materials at the nanoscale and microscale materials as model systems for stem cell and human biology materials for tissue engineering and regenerative medicine (Nano)materials and (nano)systems for therapeutic delivery interactions at the biointerface biologically inspired and biomimetic materials, including bio-inspired self-assembly systems and cell-inspired synthetic tools next-generation biomaterials tools and methods











![Sodium 4-{[(2R,3R)-2-[(dichloroacetyl)amino]-3-hydroxy-3-(4-nitrophenyl)propyl]oxy}-4-oxobutanoate structure Sodium 4-{[(2R,3R)-2-[(dichloroacetyl)amino]-3-hydroxy-3-(4-nitrophenyl)propyl]oxy}-4-oxobutanoate structure](https://cnstatic.chemtradehub.com/structs/982/982-57-0-e747.webp)

![[4-(Hydroxymethyl)phenyl]acetic acid structure [4-(Hydroxymethyl)phenyl]acetic acid structure](https://cnstatic.chemtradehub.com/structs/734/73401-74-8-5a54.webp)
