An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity
文献信息
Sara M. Elkashef, Mark Sutherland, Laurence H. Patterson, Paul M. Loadman, Robert A. Falconer
The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB–DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.
期刊推荐

Helvetica Chimica Acta

Journal of Heterocyclic Chemistry

Planta Medica

Pure and Applied Chemistry

Organic Preparations and Procedures International

Fibre Chemistry

Kinetics and Catalysis

European Journal of Wood and Wood Products

Journal of Physics and Chemistry of Solids

Proceedings of the National Academy of Sciences of the United States of America
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