A new colorimetric assay method for the detection of anti-hepatitis C virus antibody with high sensitivity

文献信息

发布日期 2019-09-05

DOI 10.1039/C9AN01466G

影响因子 4.616

作者

Wenxin Chai, Liu Shi, Hai Shi, Anzhi Sheng, Jie Yang

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摘要

The early detection of low abundance anti-hepatitis C virus antibody (anti-HCV Ab) is critical for efficient diagnosis and treatment of HCV infection. In this work, a new colorimetric assay method has been proposed for the sensitive detection of anti-HCV Ab. In this method, the antibody-induced DNA strand displacement and the resulting rolling circle amplification (RCA) are integrated to generate a large amount of tandemly repeated G-quadruplex DNAzymes on the arm of the “Y”-shaped antibody. Consequently, oxidation of 3,3′,5,5′-tetramethylbenzidine can be extensively catalyzed by the peroxidase-mimicking DNAzymes. Therefore, the readout signal can be greatly amplified. Further studies reveal that 0.998 pM anti-HCV Ab can be detected by this newly developed assay method. Moreover, the strategy proposed in this method can be adapted for the detection of other antibodies or bivalent targets.

期刊推荐

European Journal of Organic Chemistry

Molecules

Photochemical & Photobiological Sciences

Mini-Reviews in Medicinal Chemistry

Green Chemistry

Current Pharmaceutical Biotechnology

Lab on a Chip

Faraday Discussions

Advanced Engineering Materials

Foundations of Chemistry

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

A new colorimetric assay method for the detection of anti-hepatitis C virus antibody with high sensitivity

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