Engineering DNAzyme cascade for signal transduction and amplification

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发布日期 2020-01-10

DOI 10.1039/C9AN02003A

影响因子 4.616

作者

Jing Li, Ke Quan, Yanjing Yang, Xiaohai Yang, Xiangxian Meng, Jin Huang, Kemin Wang

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摘要

Inspired by the natural enzyme cascade reaction, a multiple DNAzyme cascade platform is engineered to imitate the intracellular process of signal transduction and signal amplification. In this design, when particular stimuli appear, an activated upstream DNAzyme will cleave a well-designed intermediary S1, releasing a downstream DNAzyme that can cleave the reporter substrate S2 to output signals. Thus, the signal is passed from the upstream DNAzyme to the downstream DNAzyme through a well-designed intermediary, accomplishing signal transduction and signal amplification. According to the experimental results, the DNAzyme cascades are capable of improving sensitivity for bioassays compared with that for single DNAzyme-based biocatalysis, which holds promise for potential applications, such as biomolecular computing, logic circuits and precision medicine.

期刊推荐

Electroanalysis

Biocatalysis and Biotransformation

NDT & E International

Journal of Chemical Sciences

Journal of the Indian Institute of Science

Journal of Asian Natural Products Research

Chinese Journal of Chemistry

Critical Reviews in Solid State and Materials Sciences

Bioorganic & Medicinal Chemistry Letters

Medicinal Chemistry Research

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Engineering DNAzyme cascade for signal transduction and amplification

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