Calorimetric, spectroscopic and computational investigation of DNA triplexes containing a 3′–3′ internucleoside junction

文献信息

发布日期
DOI 10.1039/A906347A
影响因子 3.676
作者


查看原文

摘要

Triple helix (triplex) formation between a 24-mer oligonucleotide double helix (duplex) and two kinds of 24-mer single strands containing a 3′–3′ phosphodiester bond was investigated by calorimetric, spectroscopic and computational techniques in order to analyze the effect of chemical modification of the third strand on the stability of DNA triplex. The duplex target is composed of two adjacent oligopurine–oligopyrimidine domains where the oligopurine sequences alternate on the two duplex strands. The third strands differ between each other from the substitution of cytosine with a thymine at the junction point. The two triplexes exhibit different CD spectra, suggesting that they have non-equivalent conformational states. Differential scanning calorimetric data show that the triplex with a cytosine–thymine junction (CT-triplex) is thermodynamically less stable than that with a cytosine–cytosine junction (CC-triplex). Molecular mechanics calculations revealed that the two triplexes have different geometries at the junction point. The thermodynamic data are successfully discussed in relation to the molecular models of the two triplexes.

相关文献

A mild method for synthesizing carboxylic acids by oxidation of aldoximes using hypervalent iodine reagents

Akira Nakamura, Hodaka Kanou, Junki Tanaka, Akira Imamiya, Tomohiro Maegawa

2018-01-02 Communication

DOI: 10.1039/C7OB02858J

Tailoring the properties of a hypoxia-responsive 1,8-naphthalimide for imaging applications

Kylie Yang, Kathryn G. Leslie, Sally Y. Kim, Bill Kalionis

2018-01-05 Paper

DOI: 10.1039/C7OB03164E

The unexpected racemization and hydrogen–deuterium exchange of the hydrogen at the α-carbon of proline analogs containing the 5-azoniaspiro[4.4]nonyl-group‡

B. Setner, M. Wierzbicka, L. Jerzykiewicz, M. Lisowski, Z. Szewczuk

2018-01-05 Paper

DOI: 10.1039/C7OB02926H

Gold catalyzed Heck-coupling of arenediazonium o-benzenedisulfonimides

Margherita Barbero, Stefano Dughera

2017-12-08 Paper

DOI: 10.1039/C7OB02624B

Hydrazone–Pd-catalyzed direct intermolecular reaction of o-alkynylphenols with allylic acetates

Kohei Watanabe, Eri Ishikawa, Chihiro Masuda

2017-12-18 Paper

DOI: 10.1039/C7OB02873C

Formal total synthesis of salvianolic acid N

Kong Wu, Zhong Pao Xie, Dong-Mei Cui, Chen Zhang

2017-12-22 Paper

DOI: 10.1039/C7OB03025H

Rh(iii)-Catalyzed ortho-C–H alkynylation of N-phenoxyacetamides with hypervalent iodine-alkyne reagents at room temperature

Sisheng Hu, Liang Lu, Tongyang Zhu, Qian Wu, Ying Chen, Jie Jack Li

2017-11-28 Communication

DOI: 10.1039/C7OB02438J

Front cover

Cover

DOI: 10.1039/C8OB90001A

PLP-independent racemization: mechanistic and mutational studies of O-ureidoserine racemase (DcsC)

Yeong-Chan Ahn, Conrad Fischer, Marco J. van Belkum, John C. Vederas

2018-01-24 Paper

DOI: 10.1039/C7OB03013D

您可能还喜欢

化合物问答

2-Bromo-4-chloro-1-(difluoromethyl)benzene(CAS号:1261476-50-9)的市场或研究趋势如何?

随着环保要求的提高和安全意识的增强,该化合物的研究和应用趋势正逐渐转向更安全、更环境友好的替代品。市场关注点主要集中在开发新型合成方法和绿色化学路径,以减少有害...

1261476-50-92-Bromo-4-chloro-1-(...
化合物问答

如何处理含有2,9 - 二苯基-1,10 - 菲罗啉(CAS号:25677-69-4)的废料?

处理含有2,9 - 二苯基 - 1,10 - 菲罗啉的废料时,应先将其收集在适当的容器中,避免与其他化学品混合。随后,可以通过水解或氧化等方法进行处理,直至达到...

25677-69-42,9-Diphenyl-1,10-ph...
化合物问答

处理(6-氯-吡嗪-3-基)-(4-乙基-哌嗪-1-基)-甲酮(CAS号:1178836-15-1)时应注意哪些实验室安全事项?

处理(6-氯-吡嗪-3-基)-(4-乙基-哌嗪-1-基)-甲酮时,应穿戴适当的个人防护装备(PPE),包括手套、护目镜和实验室外套。在通风橱中操作以确保良好的通...

1178836-15-1(6-Chloro-3-pyridazi...
化合物问答

处理(R)-2-氯-1-(2,4-二氯苯基)乙醇(CAS号:114446-57-0)时应注意哪些实验室安全事项?

在处理(R)-2-氯-1-(2,4-二氯苯基)乙醇时,应佩戴防护眼镜、实验室外套和手套,确保通风橱开启以减少接触和吸入的风险。避免直接接触皮肤和眼睛。处理过程中...

114446-57-0(1R)-2-Chloro-1-(2,4...
化合物问答

在合成中是否有3-氯-6-(3-氯哌啶-1-基)吡嗪(CAS号:1185310-37-5)的替代品?

可考虑使用类似结构的化合物作为替代品,如3-氯-6-(哌啶-1-基)吡嗪或3-氯-6-(2-氯哌啶-1-基)吡嗪,这些化合物在结构上与目标化合物相似,可能具有相...

1185310-37-53-Chloro-6-(3-chloro...
化合物问答

苯并三氮唑-5-甲酸乙酯(CAS号:73605-91-1)通常如何合成?

该化合物可以通过乙酸乙酯与5-溴-1H-苯并三氮唑的反应合成,通常在无水条件下进行。合成过程中,需要使用适当的溶剂如乙酸乙酯,并在适当的温度下反应。该反应具有较...

73605-91-1Ethyl 1H-benzotriazo...
化合物问答

什么是一水硫酸镁(CAS号:14168-73-1)?

一水硫酸镁是一种无机化合物,化学式为MgSO₄·H₂O,CAS号为14168-73-1。它由镁离子、硫酸根离子和一个结晶水分子组成,通常呈现为白色粉末或颗粒状固...

14168-73-1Magnesium sulfate hy...
化合物问答

氘代-1,3-二氯-2-丙醇(CAS号:1173020-20-6)应用于哪些行业?

氘代-1,3-二氯-2-丙醇主要应用于医药和有机合成领域,作为研究化合物的氘代替代品,用于标记和追踪反应过程。此外,在聚合物和半导体生产中也有一定的应用潜力。

1173020-20-61,3-Dichloro-2-(~2~H...
化合物问答

如何储存氰乙酸环己酯(CAS号:52688-11-6)?

氰乙酸环己酯应储存在阴凉、干燥、通风良好的环境中,远离火源和热源,防止阳光直射。储存容器应密封良好,避免与空气接触,防止发生不必要的反应。

52688-11-6Cyclohexyl cyanoacet...
化合物问答

2-碘-4-硝基苯胺(CAS号:6293-83-0)的市场或研究趋势如何?

目前,2-碘-4-硝基苯胺在医药和农药领域有一定的研究和应用,尤其是在开发新型药物和农药产品方面。然而,由于其潜在的环境和健康风险,行业正趋向于寻找更为安全和环...

6293-83-02-iodo-4-nitroanilin...

来源期刊

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
自引率: 10.3%
年发文量: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

推荐供应商

免责声明
本页面提供的学术期刊信息仅供参考和研究使用。我们与任何期刊出版商均无关联,也不处理投稿事宜。如有投稿相关咨询,请直接联系相关期刊出版商。
如发现页面信息有误,请发送邮件至 support@chemtradehub.com 联系我们。我们将及时核实并处理您的问题。