Use of inherently conducting polymers and pulsed amperometry in flow injection analysis to detect oligonucleotides
文献信息
Yanzhe Wu, Simon E. Moulton, Chee O. Too, Gordon G. Wallace, Dezhi Zhou
Pulsed amperometry has been used to detect and discriminate between different base-pair oligonucleotides using flow injection analysis with polypyrrole modified electrodes. Oligonucleotides with 20-repeat units were immobilized as the sole counter anion during polymerisation of pyrrole onto a glassy carbon (GC) electrode. The modified electrode was then used in a flow injection analysis (FIA) set-up to selectively detect complementary oligonucleotides. Results showed that complementary oligo(dG)20 and non-complementary oligonucleotides can be distinguished by using the oligo(dC)20 modified electrode.
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