Ultra-smooth glass channels for bioassay with motor proteins

文献信息

发布日期 2004-08-09

DOI 10.1039/B409223F

影响因子 4.616

作者

查看原文

摘要

Optically-flat glass channels were fabricated for the non-fluorescent observation of bio-molecules. Two bioassays of motor proteins were successfully performed, in which nanoscale beads and microtubules were clearly observed by a differential interference contrast (DIC) microscope and a dark-field microscope, respectively. The concentration of hydrofluoric acid (HF) was optimized to obtain an optically flat surface which was evaluated by AFM, SEM and dark-field microscopy. A glass channel was etched using a poly(dimethyl siloxane) (PDMS) microfluidic channel as a mask and sealed with a PDMS-coated coverslip. The channel volume of 2–3 µl realized the drastic reduction of proteins required per assay compared with a conventional flow cell method requiring 20 µl.

期刊推荐

Drug and Chemical Toxicology

Journal of Dispersion Science and Technology

Pharmacology & Therapeutics

Progress in Materials Science

Tetrahedron

Annual Review of Public Health

Studies in Conservation

Bioorganic Chemistry

Dental Materials

Tetrahedron Letters

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Ultra-smooth glass channels for bioassay with motor proteins

文献信息

期刊推荐

相关文献

Biobased polymers derived from itaconic acid bearing clickable groups with potent antibacterial activity and negligible hemolytic activity

A pillar[5]arene-based 3D polymer network for efficient iodine capture in aqueous solution

Hollow microspherical carbazole-based conjugated polymers by photoinduced step-growth polymerization

Back cover

The synergistic effect of rigid and flexible substituents on insertion polymerization with α-diimine nickel and palladium catalysts

Modeling ultrasound-induced molecular weight decrease of polymers with multiple scissile azo-mechanophores

Construction of biodegradable core cross-linked nanoparticles from near infrared dyes encoded in polyprodrug amphiphiles and investigation of their synergistic anticancer activity

A quinine-based quaternized polymer: a potent scaffold with bactericidal properties without resistance

Polyolefin graft copolymers through a ring-opening metathesis grafting through approach

The synthesis of thermoresponsive POSS-based eight-arm star poly(N-isopropylacrylamide): A comparison between Z-RAFT and R-RAFT strategies

您可能还喜欢

6-氯-2H-1,4-苯并噁嗪-3(4H)-酮(CAS号：7652-29-1)应用于哪些行业？

活性氧化铝(CAS号：1302-74-5)应用于哪些行业？

什么是硅胶(CAS号：112926-00-8)？

二乙基甲基一氢硅烷(CAS号：760-32-7)的主要用途是什么？

在合成中是否有N-花生四烯酰基甘氨酸(CAS号：179113-91-8)的替代品？

在合成中是否有1-(4-甲氧基苯基)丙烷-1,2-二酮(CAS号：10557-27-4)的替代品？

N-(4-氨基-1-苄基-3-羟基-5-苯基戊基)-3-甲基-2-(2-氧代四氢嘧啶-1-基)-丁酰胺 5-氧代吡咯烷-2-甲酸(CAS号：192726-06-0)通常如何合成？

(S)-2-氨基-3-喹啉-2-丙酸(CAS号：161513-46-8)的市场或研究趋势如何？

核黄素磷酸钠(CAS号：130-40-5)安全吗？

盐酸丙胺卡因杂质A(EP) 标准品(CAS号：19281-31-3)通常如何合成？

来源期刊

Analyst

推荐化合物

Direct Blue 86

3-bromo-5-(trifluoromethyl)benzoic acid

4-Methoxy-3-nitroaniline

4-{2-[4-(2-Methyl-2-propanyl)phenyl]ethoxy}quinazoline

(2,2-Dichlorocyclopropyl)benzene

推荐供应商