Construction of an electrochemical DNA chip for simultaneous genotyping of single nucleotide polymorphisms

文献信息

发布日期 2005-03-22

DOI 10.1039/B414030N

影响因子 4.616

作者

Masayoshi Takahashi, Jun Okada, Keiko Ito, Michie Hashimoto, Koji Hashimoto, Yuri Yoshida, Yasuhiro Furuichi, Yasuhiko Ohta, Sunji Mishiro, Nobuhiro Gemma

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摘要

An electrochemical DNA chip was constructed for simultaneous genotyping of single nucleotide polymorphisms (SNPs) using genomic DNA extracted from blood samples. This chip consisted of electrodes located on a single piece of substrate and allele-specific oligonucleotide probes on the electrodes. As a first application, the 4 SNPs (MxA[−88], MxA[−123], MBL[X/Y], and MBL[A/B]), which have association with the efficacy of interferon therapy for HCV patient, were genotyped on the new DNA chip. Following hybridization of PCR products containing the 4 types of fragments, washing, bisbenzimide H33258 (Hoechst 33258) reaction and electrochemical analyses, 59 blood samples were genotyped by the chip method simultaneously. All procedures were completed within 2 h and the results were 100% concordant with those by the direct sequence method. The electrochemical DNA chip is expected to be a practical tool for SNPs genotyping.

期刊推荐

Heteroatom Chemistry

Acta Metallurgica Sinica-English Letters

Main Group Chemistry

Journal of the Indian Institute of Science

Bioorganic & Medicinal Chemistry

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Journal of Asian Natural Products Research

Herald of the Russian Academy of Sciences

Critical Reviews in Solid State and Materials Sciences

来源期刊

Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Construction of an electrochemical DNA chip for simultaneous genotyping of single nucleotide polymorphisms

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