Optimization of LC-DRC-ICP-MS for the speciation of selenotrisulfides with simultaneous detection of sulfur and selenium as oxides combined with determination of elemental and isotope ratios

文献信息

发布日期 2005-12-16
DOI 10.1039/B514633J
影响因子 4.023
作者

Stefan Stürup, Lars Bendahl, Bente Gammelgaard


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摘要

A LC-DRC-ICP-MS method for the simultaneous detection of selenium and sulfur in the selenotrisulfides selenocysteineglutathione (Cys-Se-SG) and selenodiglutathione (GS-Se-SG) is described. Both sulfur and selenium are reacted with oxygen in the dynamic reaction cell (DRC) and detected as oxides. The selenotrisulfides were separated applying a 30 min gradient liquid chromatographic (LC) method with a formic acid/methanol eluent. The detection limits for sulfur (as 32S16O+) and selenium (as 80Se16O+) in the chromatographic system were 4.0 and 0.2 μg L−1 (100 and 5 ng in absolute mass units), respectively. S/Se elemental ratios in the 1.9–2.1 range, close to the theoretical value of 2.0, was found for both selenotrisulfides along with accurate (<2.5% deviation) and precise (∼1% RSD) sulfur and selenium isotope ratios. This emphasises the potential of the developed method for the speciation of sulfur and/or selenium compounds combined with measurement of elemental and isotope ratios in the characterisation and quantification of biomolecules and drug metabolites.

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来源期刊

Journal of Analytical Atomic Spectrometry

Journal of Analytical Atomic Spectrometry
CiteScore: 6.2
自引率: 25.8%
年发文量: 254

The Journal of Analytical Atomic Spectrometry (JAAS) is the central journal for publishing innovative research on fundamentals, instrumentation, and methods in the determination, speciation and isotopic analysis of (trace) elements within all fields of application. This includes, but is not restricted to, the most recent progress, developments and achievements in all forms of atomic and elemental detection, isotope ratio determination, molecular analysis, plasma-based analysis and X-ray techniques. The journal welcomes full papers, communications, technical notes, critical and tutorial review articles, editorials, and comments, in addition to the Atomic Spectrometry Updates (ASU) literature reviews that are prepared by an expert panel. Submissions are welcome in the following areas, but note this list reflects the current scope and authors are strongly encouraged to contact the Editorial team if they believe that their work offers potentially new and emerging research relevant to the journal remit: Fundamental studies in the following. New and existing sources for atomic emission, absorption, fluorescence and mass spectrometry and those that provide both atomic and molecular information Sample introduction techniques for solids, liquids, gases Improvements in sensitivity, selectivity, precision, accuracy and/or robustness Isotope ratio measurements, including techniques for improving precision and mass bias correction Single channel and multichannel simultaneous detection systems Chemometrics, statistics, calibration techniques and internal standardisation Theoretical and numerical modelling of fundamental processes related to all of the above methodologies Novel or improved methodologies in areas of application including, but not limited to the following. Biosciences, including elemental, speciation and isotopic analysis in biological systems, immunoassays based on metal-labeled antibodies, bio-imaging, and nanoparticle toxicology Geochemistry Environmental science Materials science, including engineered nanoparticles and quantum dots Metrology, including reference materials Forensic analysis Food and agricultural sciences Energy Archaeometry Molecular analysis. Molecular sources for elemental and isotopic analysis Atomic sources for molecular analysis Atomic and molecular techniques simultaneously used for complementary chemical information All contributions are judged on originality and quality of scientific content, and appropriateness of length to content of new science.

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