A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

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发布日期 2014-12-10

DOI 10.1039/C4AN02064B

影响因子 4.616

作者

Hisakage Funabashi, Keisuke Nakatsuka, Akio Kuroda

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摘要

A DNA nano-tweezer (DNA-NT) structure-based target mRNA detection probe, which uses fluorescence resonance energy transfer (FRET) as a detection signal and works as a single molecule, has been developed. This FRET-paired fluorescent dye-modified DNA-NT, self-assembled from three single-stranded DNAs, alters its structure from open to closed states and produces a FRET signal in response to in vitro transcripts of Hes-1 mRNA. Our results showed that the FRET-based DNA-NT detected both GLUT1 mRNA as a pre-fixed target mRNA model and Hes-1 mRNA as a model expressed inside a living cell. These results confirm the feasibility of using the FRET-based DNA-NT for imaging analysis of target mRNA.

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Advanced Engineering Materials

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CrystEngComm

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Journal of Enzyme inhibition and Medicinal Chemistry

Molecular Diversity

Coloration Technology

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Analyst

CiteScore: 7.8

自引率: 5.6%

年发文量: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

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