Estimating and correcting interference fringes in infrared spectra in infrared hyperspectral imaging
文献信息
发布日期
2018-09-03
DOI
10.1039/C8AN00093J
影响因子
4.616
作者
Ghazal Azarfar, Ebrahim Aboualizadeh, Nicholas M. Walter, Simona Ratti, Camilla Olivieri, Alessandra Norici, Michael Nasse, Achim Kohler, Mario Giordano
查看原文
摘要
Short-term acclimation response of individual cells of Thalassiosira weissflogii was monitored by Synchrotron FTIR imaging over the span of 75 minutes. The cells, collected from batch cultures, were maintained in a constant flow of medium, at an irradiance of 120 μmol m−2 s−1 and at 20 °C. Multiple internal reflections due to the micro fluidic channel were modeled, and showed that fringes are additive sinusoids to the pure absorption of the other components of the system. Preprocessing of the hyperspectral cube (x, y, Abs(λ)) included removing spectral fringe using an EMSC approach. Principal component analysis of the time series of hyperspectral cubes showed macromolecular pool variations (carbohydrates, lipids and DNA/RNA) of less than 2% after fringe correction.
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来源期刊
Analyst
CiteScore:
7.8
自引率:
5.6%
年发文量:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
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