Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers
文献信息
Zhiwen Liu, Liang Ma, Liping Zhang, Wenjun Zhang, Yiguang Zhu, Yuchan Chen, Weimin Zhang, Changsheng Zhang
The halogenase gene spmH was putatively involved in the biosynthesis of spiroindimicins/indimicins (SPMs/IDMs), a group of chlorinated tryptophan dimers (TDs) from deep-sea-derived Streptomyces sp. SCSIO 03032. Inactivation of spmH led to six deschloro-analogues of TDs, including four new compounds SPMs G (1) and H (2), and IDMs F (3) and G (4). The structures and absolute configurations of 1–4 were unambiguously determined by the combination of extensive spectroscopic analysis, single-crystal X-ray diffraction and quantum chemical ECD calculations. Compounds 1 and 2 exhibited moderate cytotoxic activities against four cancer cell lines. Additionally, SpmH was biochemically characterized in vitro as an L-tryptophan 5-halogenase.
期刊推荐

European Journal of Wood and Wood Products

Science Progress

Planta Medica

Organic Preparations and Procedures International

Proceedings of the National Academy of Sciences of the United States of America

Journal of Physics and Chemistry of Solids

Israel Journal of Chemistry

Journal of Medicinal Chemistry

Pure and Applied Chemistry

Kinetics and Catalysis
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Organic & Biomolecular Chemistry

Organic & Biomolecular Chemistry (OBC) publishes original and high impact research and reviews in organic chemistry. We welcome research that shows new or significantly improved protocols or methodologies in total synthesis, synthetic methodology or physical and theoretical organic chemistry as well as research that shows a significant advance in the organic chemistry or molecular design aspects of chemical biology, catalysis, supramolecular and macromolecular chemistry, theoretical chemistry, mechanism-oriented physical organic chemistry, medicinal chemistry or natural products. Articles published in the journal should report new work which makes a highly-significant impact in the field. Routine and incremental work is generally not suitable for publication in the journal. More details about key areas of our scope are below. In all cases authors should include in their article clear rationale for why their research has been carried out.




