A gene-activating skin substitute comprising PLLA/POSS nanofibers and plasmid DNA encoding ANG and bFGF promotes in vivo revascularization and epidermalization

文献信息

发布日期 2018-09-28
DOI 10.1039/C8TB02006J
影响因子 6.331
作者

Dongwei Wu, Jianwang Tan, Zhibin Liu


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摘要

Vascularization of deep skin defects is a key challenge for the healing of tissue with blood vessel structures. In this research, to accelerate wound regeneration, complexes of trimethyl chitosan chloride (TMC) and multiple plasmid DNAs (pDNAs) encoding angiopoietin (ANG) and basic fibroblast growth factor (bFGF) were integrated into porous nanofibers of poly(L-lactic acid) (PLLA)/polyhedral oligomeric silsesquioxane (POSS); these nanofibers were used to treat deep skin defects. The pDNAs were stably released in a sustained manner from the PLLA/POSS porous nanofibrous scaffold. The PLLA/POSS/pDNA nanofibers showed a notable positive influence on the transfection of human umbilical vein endothelial cells (HUVECs) in vitro, and the PLLA/POSS/pANG/pbFGF (Fab) composite nanofibers showed a significantly higher expression of ANG and vinculin than the nanofibers without pDNA or the nanofibers loaded with only pANG or pbFGF. The four different composite nanofibers were further transplanted onto deep burn wounds on the backs of rats in vivo. The results indicated that the Fab nanofibers had observably higher neovasculature regeneration. This finding revealed that Fab not only prevented the inflammatory response but also accelerated wound regeneration. All of the results showed that pANG and pbFGF exhibited positive effects in promoting tissue repair. In particular, bFGF can guide perithelial cells to form a cellular layer around new capillaries, ensuring vascular maturation and preventing vascular degradation. Therefore, an even higher level of vascularization can be realized when ANG and bFGF are both present to achieve desirable interactions for wound repair.

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来源期刊

Journal of Materials Chemistry B

Journal of Materials Chemistry B
CiteScore: 12
自引率: 4.9%
年发文量: 831

Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. The journals have a strong history of publishing quality reports of interest to interdisciplinary communities and providing an efficient and rigorous service through peer review and publication. The journals are led by an international team of Editors-in-Chief and Associate Editors who are all active researchers in their fields. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C. More than one Journal of Materials Chemistry journal may be suitable for certain fields and researchers are encouraged to submit their paper to the journal that they feel best fits for their particular article. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive. Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices image block All articles published in Journal of Materials Chemistry B from 2019 onwards will be indexed in MEDLINE®. Articles that primarily focus on providing insight into the underlying science and performance of biomaterials within a biological environment are more suited to our companion journal, Biomaterials Science.

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