Efficient bioconversion of crude glycerol from biodiesel to optically pure d-lactate by metabolically engineered Escherichia coli
文献信息
Kang-ming Tian, Dan-dan Niu, Govender Algasan, Suren Singh, Zheng-xiang Wang
Biodiesel has attracted considerable attention as one of the best choices among alternative and renewable fuels. Large quantities of crude glycerol are produced as a main co-product with increasing biodiesel production. Currently, the problem of waste glycerol utilization needs to be crucially addressed, not only for environmental protection but also for the economy of the biodiesel industry. In this paper, the use of crude glycerol for the production of D-lactate by engineered Escherichia coli was investigated. Engineered E. coli B0013-070 with a homolactic pathway for D-lactate synthesis by elimination of byproduct pathways (ethanol, succinate, formate and acetate) could convert 20 g L−1 of crude glycerol to 13.6 g L−1 of D-lactate with a yield of 0.67 g g−1 glycerol. Overexpression of D-lactate dehydrogenase by a low-copy vector in E. coli B0013-070 resulted in the increased production and yield of D-lactate, in which 14.5 g L−1 of D-lactate was produced with a yield of 0.72 g g−1 glycerol from crude glycerol. The effect of temperature on D-lactate fermentation by the engineered strain E. coli B0013-070-pTHldhA was also investigated, and 34 °C and 40 °C were found to be the optimal temperatures for cell growth and lactate production, respectively. The engineered strain B0013-070-pTHldhA produced 100.3 g L−1 of D-lactate with 99.97% optical purity from 531.5 g of crude glycerol with an overall productivity of 2.78 g L−1 h−1 and a yield of 75.4 g per 100 g glycerol (0.77 mol mol−1) using two phase fermentation combined with a temperature shifting strategy in a 7 L bioreactor. In summary, this paper shows that crude glycerol could be directly converted to D-lactate without any prior purification.
期刊推荐

Journal of the Indian Institute of Science

NDT & E International

Bioorganic & Medicinal Chemistry Letters

Atomization and Sprays

Chinese Journal of Chemistry

Critical Reviews in Solid State and Materials Sciences

Acta Metallurgica Sinica-English Letters

Heteroatom Chemistry

Journal of Chemical Sciences

Main Group Chemistry
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Green Chemistry

Green Chemistry provides a unique forum for the publication of innovative research on the development of alternative green and sustainable technologies. The scope of Green Chemistry is based on, but not limited to, the definition proposed by Anastas and Warner (Green Chemistry: Theory and Practice, P T Anastas and J C Warner, Oxford University Press, Oxford, 1998). Green chemistry is the utilisation of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Green Chemistry is at the frontiers of this continuously-evolving interdisciplinary science and publishes research that attempts to reduce the environmental impact of the chemical enterprise by developing a technology base that is inherently non-toxic to living things and the environment. Submissions on all aspects of research relating to the endeavour are welcome. The journal publishes original and significant cutting-edge research that is likely to be of wide general appeal. To be published, work must present a significant advance in green chemistry. Papers must contain a comparison with existing methods and demonstrate advantages over those methods before publication can be considered. For more information please see this Editorial. Coverage includes the following, but is not limited to: Design (e.g. biomimicry, design for degradation/recycling/reduced toxicity…) Reagents & Feedstocks (e.g. renewables, CO2, solvents, auxiliary agents, waste utilization…) Synthesis (e.g. organic, inorganic, synthetic biology…) Catalysis (e.g. homogeneous, heterogeneous, enzyme, whole cell…) Process (e.g. process design, intensification, separations, recycling, efficiency…) Energy (e.g. renewable energy, fuels, photovoltaics, fuel cells, energy storage, energy carriers…) Applications (e.g. electronics, dyes, consumer products, coatings, pharmaceuticals, preservatives, building materials, chemicals for industry/agriculture/mining…) Impact (e.g. safety, metrics, LCA, sustainability, (eco)toxicology…) Green chemistry is, by definition, a continuously-evolving frontier. Therefore, the inclusion of a particular material or technology does not, of itself, guarantee that a paper is suitable for the journal. To be suitable, the novel advance should have the potential for reduced environmental impact relative to the state of the art. Green Chemistry does not normally deal with research associated with 'end-of-pipe' or remediation issues.




