Efficient regio- and stereo-selective C–H bond hydroxylation of steroids using an engineered heme-thiolate peroxygenase biocatalyst

文献信息

发布日期 2023-10-05
DOI 10.1039/D3CY01223A
影响因子 6.119
作者

Jinia Akter, Eva F. Hayball, Stephen G. Bell


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摘要

A crucial reaction in chemical synthesis is the activation of unactivated carbon–hydrogen bonds in complex molecules. We demonstrate the regio- and stereo-selective hydroxlation of the steroids progesterone and androstenedione using the peroxygenase activity of an engineered bacterial cytochrome P450 enzyme, CYP154C8. By replacing a single amino acid of the I-helix we change this monooxygenase enzyme into a peroxygenase, enabling the efficient and selective biocatalytic formation of the 16α-hydroxy steroid metabolite.

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来源期刊

Catalysis Science & Technology

Catalysis Science & Technology
CiteScore: 5.91
自引率: 4.5%
年发文量: 600

Catalysis Science & Technology is committed to publishing research reporting high-quality, cutting-edge developments across the catalysis community at large. The journal places equal focus on publications from the heterogeneous, homogeneous, thermo-, electro-, photo-, organo- and biocatalysis communities. Works published in the journal feature a balanced mix of fundamental, technology-oriented, experimental, computational, digital and data-driven original research, thus appealing to catalysis practitioners in both academic and industrial environments. Original research articles published in the journal must demonstrate new catalytic discoveries and/or methodological advances that represent a significant advance on previously published work, from the molecular to the process scales. We welcome rigorous research in a wide range of timely or emerging applications related to the environment, health, energy and materials. Catalysis Science & Technology publishes Communications, Articles, Reviews and Perspectives. More details regarding manuscript types may be found in the Information for Authors section.

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